CXCL9 Recombinant Rabbit Monoclonal Antibody [PSH12-34]

☑ Cell treatment (CT)

Western blot analysis of CXCL9 on different lysates with Rabbit anti-CXCL9 antibody (HA723608) at 1/1,000 dilution.

Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 100ng/mL IFN-gamma for 24 hours then treated with 300ng/mL BFA for 20 hours cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 14 kDa
Observed band size: 17 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723608) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

☑ Cell treatment (CT)

Immunocytochemistry analysis of THP-1 cells untreated / treated with 100ng/mL IFN-gamma for 24 hours then treated with 300ng/mL BFA for 20 hours labeling CXCL9 with Rabbit anti-CXCL9 antibody (HA723608) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CXCL9 antibody (HA723608) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.