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ALP Recombinant Rabbit Monoclonal Antibody [PSH14-35]

Usd: 385

Specification

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Catalog# HA723629

ALP Recombinant Rabbit Monoclonal Antibody [PSH14-35]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

ALP Recombinant Rabbit Monoclonal Antibody [PSH14-35]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ALP aa 1-364.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 39 kDa

Positive Control

Saos-2 cell lysate, RD cell lysate, U-2 OS cell lysate, 293T cell lysate, C2C12 cell lysate, L6 cell lysate, Mouse heart tissue lysate, Rat heart tissue lysate.

Conjugation

unconjugated

Clone Number

PSH14-35

Reactivity Data

WB IP
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IP

  • 1-2μg/sample

Target

Function

Actin-associated LIM protein (ALP), also known as PDZ and LIM domain protein 3 is a protein that in humans is encoded by the PDLIM3 gene. ALP is highly expressed in cardiac and skeletal muscle, where it localizes to Z-discs and intercalated discs. ALP functions to enhance the crosslinking of actin by alpha-actinin-2 and also appears to be essential for right ventricular chamber formation and contractile function.

Background References

1. Zhang J et al. PDLIM3 supports hedgehog signaling in medulloblastoma by facilitating cilia formation. Cell Death Differ. 2023 May

2. Liu M et al. PDLIM3 knockdown promotes ferroptosis in endometriosis progression via inducing Gli1 degradation and blocking Hedgehog signaling pathway. J Assist Reprod Genet. 2024 Aug

Subcellular Location

Cytoplasm, myofibril, sarcomere, Z line.

UNIPROT

SwissProt: Q53GG5 Human

SwissProt: O70209 Mouse

SwissProt: Q66HS7 Rat

Synonyms

Actinin associated LIM protein antibody

Actinin-associated LIM protein antibody

Alpha actinin 2 associated LIM protein antibody

Alpha-actinin-2-associated LIM protein antibody

DKFZp686L0362 antibody

Enigma homolog antibody

PDLI3_HUMAN antibody

PDLIM 3 antibody

PDLIM3 antibody

PDZ and LIM domain 3 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of ALP on different lysates with Rabbit anti-ALP antibody (<a href="/products/HA723629" style="font-weight: bold;text-decoration: underline;">HA723629</a>) at 1/5,000 dilution.<br /><br />Lane 1: Saos-2 cell lysate<br />Lane 2: RD cell lysate<br />Lane 3: U-2 OS cell lysate<br />Lane 4: 293T cell lysate<br />Lane 5: C2C12 cell lysate<br />Lane 6: L6 cell lysate<br />Lane 7: Mouse heart tissue lysate<br />Lane 8: Mouse kidney tissue lysate (negative)<br />Lane 9: Rat heart tissue lysate<br />Lane 10: Rat kidney tissue lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 39 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723629" style="font-weight: bold;text-decoration: underline;">HA723629</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of ALP on different lysates with Rabbit anti-ALP antibody (HA723629) at 1/5,000 dilution.

    Lane 1: Saos-2 cell lysate
    Lane 2: RD cell lysate
    Lane 3: U-2 OS cell lysate
    Lane 4: 293T cell lysate
    Lane 5: C2C12 cell lysate
    Lane 6: L6 cell lysate
    Lane 7: Mouse heart tissue lysate
    Lane 8: Mouse kidney tissue lysate (negative)
    Lane 9: Rat heart tissue lysate
    Lane 10: Rat kidney tissue lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 39 kDa
    Observed band size: 35 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723629) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • ALP was immunoprecipitated from 0.2 mg Saos-2 cell lysate with <a href="/products/HA723629" style="font-weight: bold;text-decoration: underline;">HA723629</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA723629" style="font-weight: bold;text-decoration: underline;">HA723629</a> at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: Saos-2 cell lysate (input)<br />Lane 2: <a href="/products/HA723629" style="font-weight: bold;text-decoration: underline;">HA723629</a> IP in Saos-2 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA723629" style="font-weight: bold;text-decoration: underline;">HA723629</a> in Saos-2 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 46 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    ALP was immunoprecipitated from 0.2 mg Saos-2 cell lysate with HA723629 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723629 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: Saos-2 cell lysate (input)
    Lane 2: HA723629 IP in Saos-2 cell lysate
    Lane 3: Rabbit IgG instead of HA723629 in Saos-2 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 46 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation