Skip to content

HUWE1 Recombinant Rabbit Monoclonal Antibody [PSH14-37]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA723631

HUWE1 Recombinant Rabbit Monoclonal Antibody [PSH14-37]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

HUWE1 Recombinant Rabbit Monoclonal Antibody [PSH14-37]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human HUWE1 aa 3,950-4,374.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 482 kDa

Positive Control

HeLa cell lysate, SW480 cell lysate, A549 cell lysate, MOLT-4 cell lysate, 293T cell lysate, Jurkat cell lysate, MCF7 cell lysate, F9 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human brain tissue, human heart tissue, mouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue.

Conjugation

unconjugated

Clone Number

PSH14-37

Reactivity Data

WB IHC-P
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000-1:20,000

  • IHC-P

  • 1:50-1:100

Target

Function

E3 ubiquitin-protein ligase HUWE1 is an enzyme that in humans is encoded by the HUWE1 gene. It performs the third step (ligation) in binding ubiquitin to proteins in a process called ubiquitination which tags the proteins for disposal. Human genetic studies that implicate HUWE1 in intellectual disability. Additional research suggests HUWE1 has implications in cancer research.

Background References

1. Guo Y et al. HUWE1 mediates inflammasome activation and promotes host defense against bacterial infection. J Clin Invest. 2020 Dec

2. Wu Y et al. Ubiquitin ligase E3 HUWE1/MULE targets transferrin receptor for degradation and suppresses ferroptosis in acute liver injury. Cell Death Differ. 2022 Sep

Subcellular Location

Cytoplasm, Nucleus, Mitochondrion.

UNIPROT

SwissProt: Q7Z6Z7 Human

SwissProt: Q7TMY8 Mouse

SwissProt: P51593 Rat

Synonyms

ARF binding protein 1 antibody

ARF BP1 antibody

ARF-binding protein 1 antibody

ARF-BP1 antibody

ARFBP1 antibody

BJ-HCC-24 tumor antigen antibody

E3 ubiquitin protein ligase HUWE1 antibody

E3 ubiquitin-protein ligase HUWE1 antibody

HECT antibody

HECT domain protein LASU1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of HUWE1 on different lysates with Rabbit anti-HUWE1 antibody (<a href="/products/HA723631" style="font-weight: bold;text-decoration: underline;">HA723631</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: SW480 cell lysate (20 µg/Lane)<br />Lane 3: A549 cell lysate (low expression) (20 µg/Lane)<br />Lane 4: MOLT-4 cell lysate (20 µg/Lane)<br />Lane 5: 293T cell lysate (20 µg/Lane)<br />Lane 6: Jurkat cell lysate (20 µg/Lane)<br />Lane 7: MCF7 cell lysate (20 µg/Lane)<br />Lane 8: F9 cell lysate (20 µg/Lane)<br />Lane 9: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 10: PC-12 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 482 kDa<br />Observed band size: 482 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />3-8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723631" style="font-weight: bold;text-decoration: underline;">HA723631</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of HUWE1 on different lysates with Rabbit anti-HUWE1 antibody (HA723631) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: SW480 cell lysate (20 µg/Lane)
    Lane 3: A549 cell lysate (low expression) (20 µg/Lane)
    Lane 4: MOLT-4 cell lysate (20 µg/Lane)
    Lane 5: 293T cell lysate (20 µg/Lane)
    Lane 6: Jurkat cell lysate (20 µg/Lane)
    Lane 7: MCF7 cell lysate (20 µg/Lane)
    Lane 8: F9 cell lysate (20 µg/Lane)
    Lane 9: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 10: PC-12 cell lysate (20 µg/Lane)

    Predicted band size: 482 kDa
    Observed band size: 482 kDa

    Exposure time: 25 seconds; ECL: K1801;

    3-8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723631) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of HUWE1 on different lysates with Rabbit anti-HUWE1 antibody (<a href="/products/HA723631" style="font-weight: bold;text-decoration: underline;">HA723631</a>) at 1/20,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 2: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 482 kDa<br />Observed band size: 482 kDa<br /><br />Exposure time: 48 seconds; ECL: K1801;<br /><br />3-8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723631" style="font-weight: bold;text-decoration: underline;">HA723631</a>) at 1/20,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of HUWE1 on different lysates with Rabbit anti-HUWE1 antibody (HA723631) at 1/20,000 dilution.

    Lane 1: Mouse brain tissue lysate (40 µg/Lane)
    Lane 2: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 482 kDa
    Observed band size: 482 kDa

    Exposure time: 48 seconds; ECL: K1801;

    3-8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723631) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-HUWE1 antibody (<a href="/products/HA723631" style="font-weight: bold;text-decoration: underline;">HA723631</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723631" style="font-weight: bold;text-decoration: underline;">HA723631</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-HUWE1 antibody (HA723631) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723631) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-HUWE1 antibody (<a href="/products/HA723631" style="font-weight: bold;text-decoration: underline;">HA723631</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723631" style="font-weight: bold;text-decoration: underline;">HA723631</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-HUWE1 antibody (HA723631) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723631) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-HUWE1 antibody (<a href="/products/HA723631" style="font-weight: bold;text-decoration: underline;">HA723631</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723631" style="font-weight: bold;text-decoration: underline;">HA723631</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-HUWE1 antibody (HA723631) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723631) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-HUWE1 antibody (<a href="/products/HA723631" style="font-weight: bold;text-decoration: underline;">HA723631</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723631" style="font-weight: bold;text-decoration: underline;">HA723631</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-HUWE1 antibody (HA723631) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723631) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-HUWE1 antibody (<a href="/products/HA723631" style="font-weight: bold;text-decoration: underline;">HA723631</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723631" style="font-weight: bold;text-decoration: underline;">HA723631</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-HUWE1 antibody (HA723631) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723631) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-HUWE1 antibody (<a href="/products/HA723631" style="font-weight: bold;text-decoration: underline;">HA723631</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723631" style="font-weight: bold;text-decoration: underline;">HA723631</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-HUWE1 antibody (HA723631) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723631) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"