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RAB27B Recombinant Rabbit Monoclonal Antibody [PSH14-68]

Usd: 385

Specification

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Catalog# HA723666

RAB27B Recombinant Rabbit Monoclonal Antibody [PSH14-68]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

RAB27B Recombinant Rabbit Monoclonal Antibody [PSH14-68]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human RAB27B aa 1-218.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 25 kDa

Positive Control

U-87 MG cell lysate, Mouse stomach tissue lysate, Rat colon tissue lysate, human colon tissue, human stomach tissue.

Conjugation

unconjugated

Clone Number

PSH14-68

Reactivity Data

WB IHC-P
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Not Recommended
Rat
Tested Tested
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:50,000

  • IHC-P

  • 1:1,000

Target

Function

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in NTRK2/TRKB axonal anterograde transport by facilitating the association of NTRK2/TRKB with KLC1. May be involved in targeting uroplakins to urothelial apical membranes (By similarity).

Background References

1. Ren JG et al. RAB27B controls palmitoylation-dependent NRAS trafficking and signaling in myeloid leukemia. J Clin Invest. 2023 Jun

2. Shan J et al. Rab27b promotes endometriosis by enhancing invasiveness of ESCs and promoting angiogenesis. Am J Reprod Immunol. 2023 Sep

Subcellular Location

Membrane, Late endosome.

UNIPROT

SwissProt: O00194 Human

SwissProt: Q99P58 Mouse

SwissProt: Q99P74 Rat

Synonyms

C25KG antibody

Rab27b antibody

RAB27B member RAS oncogene family antibody

RAS associated protein RAB27B antibody

Ras related protein Rab 27b antibody

Ras related protein Rab27b antibody

Ras-related protein Rab-27B antibody

RB27B_HUMAN antibody

Small GTP binding protein rab27b antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of RAB27B on different lysates with Rabbit anti-RAB27B antibody (<a href="/products/HA723666" style="font-weight: bold;text-decoration: underline;">HA723666</a>) at 1/50,000 dilution.<br /><br />Lane 1: U-87 MG cell lysate (20 µg/Lane)<br />Lane 2: Mouse stomach tissue lysate (30 µg/Lane)<br />Lane 2: Mouse skeletal muscle tissue lysate (negative) (30 µg/Lane)<br />Lane 2: Rat colon tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 25 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723666" style="font-weight: bold;text-decoration: underline;">HA723666</a>) at 1/50,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of RAB27B on different lysates with Rabbit anti-RAB27B antibody (HA723666) at 1/50,000 dilution.

    Lane 1: U-87 MG cell lysate (20 µg/Lane)
    Lane 2: Mouse stomach tissue lysate (30 µg/Lane)
    Lane 2: Mouse skeletal muscle tissue lysate (negative) (30 µg/Lane)
    Lane 2: Rat colon tissue lysate (30 µg/Lane)

    Predicted band size: 25 kDa
    Observed band size: 25 kDa

    Exposure time: 10 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723666) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-RAB27B antibody (<a href="/products/HA723666" style="font-weight: bold;text-decoration: underline;">HA723666</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723666" style="font-weight: bold;text-decoration: underline;">HA723666</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-RAB27B antibody (HA723666) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723666) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-RAB27B antibody (<a href="/products/HA723666" style="font-weight: bold;text-decoration: underline;">HA723666</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723666" style="font-weight: bold;text-decoration: underline;">HA723666</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-RAB27B antibody (HA723666) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723666) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-RAB27B antibody (<a href="/products/HA723666" style="font-weight: bold;text-decoration: underline;">HA723666</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723666" style="font-weight: bold;text-decoration: underline;">HA723666</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-RAB27B antibody (HA723666) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723666) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of RAB27B on different lysates with Rabbit anti-RAB27B antibody (<a href="/products/HA723666" style="font-weight: bold;text-decoration: underline;">HA723666</a>) at 1/5,000 dilution.<br /><br />Lane 1: His-tagged RARB27A recombinant protein<br />Lane 2: His-tagged RARB27B recombinant protein<br /><br />Lysates/proteins at 20 ng/Lane.<br /><br />Exposure time: 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723666" style="font-weight: bold;text-decoration: underline;">HA723666</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RAB27B on different lysates with Rabbit anti-RAB27B antibody (HA723666) at 1/5,000 dilution.

    Lane 1: His-tagged RARB27A recombinant protein
    Lane 2: His-tagged RARB27B recombinant protein

    Lysates/proteins at 20 ng/Lane.

    Exposure time: 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723666) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation