Biotin Conjugated Human Myeloperoxidase Recombinant Rabbit Monoclonal Antibody [PSH14-79] - Detector
Overview
Product Name
Biotin Conjugated Human Myeloperoxidase Recombinant Rabbit Monoclonal Antibody [PSH14-79] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Myeloperoxidase aa 49-745 (HA211022).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human Myeloperoxidase protein (HA211022).
Conjugation
Biotin
Clone Number
PSH14-79
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-78] to Human Myeloperoxidase antibody (Capture) (HA723673) and Recombinant Human Myeloperoxidase protein (HA211022) as the standard. The reference range value is 93.8-12,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Myeloperoxidase (MPO) is a peroxidase enzyme that in humans is encoded by the MPO gene on chromosome 17. MPO is most abundantly expressed in neutrophil granulocytes (a subtype of white blood cells), and produces hypohalous acids to carry out their antimicrobial activity, including hypochlorous acid, the sodium salt of which is the chemical in bleach. It is a lysosomal protein stored in azurophilic granules of the neutrophil and released into the extracellular space during degranulation. Neutrophil myeloperoxidase has a heme pigment, which causes its green color in secretions rich in neutrophils, such as mucus and sputum. The green color contributed to its outdated name verdoperoxidase. Immunohistochemical staining for myeloperoxidase used to be administered in the diagnosis of acute myeloid leukemia to demonstrate that the leukemic cells were derived from the myeloid lineage. Myeloperoxidase staining is still important in the diagnosis of myeloid sarcoma, contrasting with the negative staining of lymphomas, which can otherwise have a similar appearance.
Background References
1. Hu CH. et. al. Small molecule and macrocyclic pyrazole derived inhibitors of myeloperoxidase (MPO). Bioorg Med Chem Lett. 2021 Jun
2. Chen S. et. al. Targeting Myeloperoxidase (MPO) Mediated Oxidative Stress and Inflammation for Reducing Brain Ischemia Injury: Potential Application of Natural Compounds. Front Physiol. 2020 May
Subcellular Location
Lysosome.
UNIPROT
Synonyms
84 kDa myeloperoxidase antibody
89 kDa myeloperoxidase antibody
EC 1.11.1.7 antibody
EC1.11.2.2 antibody
fj80f04 antibody
MPO antibody
mpx antibody
myeloid-specific peroxidase antibody
Myeloperoxidase antibody
Myeloperoxidase heavy chain antibody
ExpandImages
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![Sandwich ELISA analysis of Human Myeloperoxidase matched pair antibodies<br /><br />Capture: <a href="/products/HA723673" style="font-weight: bold;text-decoration: underline;">HA723673</a>, Human Myeloperoxidase Rabbit mAb [PSH14-78]<br />Detector: <a href="/products/HA723674" style="font-weight: bold;text-decoration: underline;">HA723674</a>, Human Myeloperoxidase Rabbit mAb [PSH14-79]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723673" style="font-weight: bold;text-decoration: underline;">HA723673</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Myeloperoxidase protein (<a href="/products/HA211022" style="font-weight: bold;text-decoration: underline;">HA211022</a>) starting from 12,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723674" style="font-weight: bold;text-decoration: underline;">HA723674</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723675B_1.png?v=20260604174801)
Sandwich ELISA analysis of Human Myeloperoxidase matched pair antibodies
Capture: HA723673, Human Myeloperoxidase Rabbit mAb [PSH14-78]
Detector: HA723674, Human Myeloperoxidase Rabbit mAb [PSH14-79]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723673) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Myeloperoxidase protein (HA211022) starting from 12,000 pg/ml to 0 pg/ml and detect antibody (HA723674, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native Myeloperoxidase in human samples.<br /><br />Capture: <a href="/products/HA723673" style="font-weight: bold;text-decoration: underline;">HA723673</a>, Human Myeloperoxidase Rabbit mAb [PSH14-78]<br />Detector: <a href="/products/HA723674" style="font-weight: bold;text-decoration: underline;">HA723674</a>, Human Myeloperoxidase Rabbit mAb [PSH14-79]<br /><br />Interpolated concentration of native Myeloperoxidase was measured in duplicate at different sample concentrations and interpolated from the Myeloperoxidase standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Myeloperoxidase concentration was determined to be 432 ng/mL in HL-60 cell supernatant. There was no detectable signal in Hela cell supernatant.](https://storage.huabio.cn/huabio/productImg/HA723675B_2.png?v=20260604174801)
Interpolated concentrations of native Myeloperoxidase in human samples.
Capture: HA723673, Human Myeloperoxidase Rabbit mAb [PSH14-78]
Detector: HA723674, Human Myeloperoxidase Rabbit mAb [PSH14-79]
Interpolated concentration of native Myeloperoxidase was measured in duplicate at different sample concentrations and interpolated from the Myeloperoxidase standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Myeloperoxidase concentration was determined to be 432 ng/mL in HL-60 cell supernatant. There was no detectable signal in Hela cell supernatant. -
![Interpolated concentrations of spiked Myeloperoxidase in cell culture media samples.<br /><br />Capture: <a href="/products/HA723673" style="font-weight: bold;text-decoration: underline;">HA723673</a>, Human Myeloperoxidase Rabbit mAb [PSH14-78]<br />Detector: <a href="/products/HA723674" style="font-weight: bold;text-decoration: underline;">HA723674</a>, Human Myeloperoxidase Rabbit mAb [PSH14-79]<br /><br />The concentrations of Myeloperoxidase were measured in duplicates, interpolated from the Myeloperoxidase standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723675B_3.png?v=20260604174801)
Interpolated concentrations of spiked Myeloperoxidase in cell culture media samples.
Capture: HA723673, Human Myeloperoxidase Rabbit mAb [PSH14-78]
Detector: HA723674, Human Myeloperoxidase Rabbit mAb [PSH14-79]
The concentrations of Myeloperoxidase were measured in duplicates, interpolated from the Myeloperoxidase standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"