Mouse IL-3 Recombinant Rabbit Monoclonal Antibody [PSH14-86] - BSA and Azide free (Detector)
Overview
Product Name
Mouse IL-3 Recombinant Rabbit Monoclonal Antibody [PSH14-86] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse IL-3 aa 27-166 (HA211168).
Species Reactivity
Mouse
Validated Applications
ELISA(Det)
Positive Control
Recombinant Mouse IL-3 protein (HA211168).
Conjugation
unconjugated
Clone Number
PSH14-86
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-85] to Mouse IL-3 antibody (Capture) (HA723683) and Recombinant Mouse IL-3 protein (HA211168) as the standard. The reference range value is 7.8-1,000 pg/mL.
Target
Function
Interleukin 3 (IL-3) is a protein that in humans is encoded by the IL3 gene localized on chromosome 5q31.1. Sometimes also called colony-stimulating factor, multi-CSF, mast cell growth factor, MULTI-CSF, MCGF; MGC79398, MGC79399: after removal of the signal peptide sequence, the mature protein contains 133 amino acids in its polypeptide chain. IL-3 is produced as a monomer by activated T cells, monocytes/macrophages and stroma cells. The major function of IL-3 cytokine is to regulate the concentrations of various blood-cell types. It induces proliferation and differentiation in both early pluripotent stem cells and committed progenitors. It also has many more specific effects like the regeneration of platelets and potentially aids in early antibody isotype switching.
Background References
1. Hong L., Tang Y., Pan S., Xu M., Shi Y., Gao S., Sui C., He C., Zheng K., Tang R., Shi Z., Wang Q., Wang H. Interleukin 3-induced GITR promotes the activation of human basophils. Cytokine 136:155268-155268 (2020).
2. Lopez C., Zamorano P., Teuber S., Salas M., Otth C., Hidalgo M.A., Concha I., Zambrano A. Interleukin-3 Prevents Cellular Death Induced by Oxidative Stress in HEK293 Cells. J. Cell. Biochem. 118:1330-1340 (2017).
Subcellular Location
Secreted.
UNIPROT
Synonyms
Colony stimulating factor multiple antibody
Hematopoietic growth factor antibody
IL 3 antibody
IL-3 antibody
IL3 antibody
IL3_HUMAN antibody
Interleukin 3 (colony stimulating factor, multiple) antibody
Interleukin 3 antibody
Interleukin-3 antibody
Mast cell growth factor antibody
ExpandImages
-
![Sandwich ELISA analysis of Mouse IL-3 matched pair antibodies<br /><br />Capture: <a href="/products/HA723683" style="font-weight: bold;text-decoration: underline;">HA723683</a>, Mouse IL-3 Rabbit mAb [PSH14-85]<br />Detector: <a href="/products/HA723684" style="font-weight: bold;text-decoration: underline;">HA723684</a>, Mouse IL-3 Rabbit mAb [PSH14-86]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723683" style="font-weight: bold;text-decoration: underline;">HA723683</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-3 protein (<a href="/products/HA211168" style="font-weight: bold;text-decoration: underline;">HA211168</a>) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723684" style="font-weight: bold;text-decoration: underline;">HA723684</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723684_1.png?v=20260429162017)
Sandwich ELISA analysis of Mouse IL-3 matched pair antibodies
Capture: HA723683, Mouse IL-3 Rabbit mAb [PSH14-85]
Detector: HA723684, Mouse IL-3 Rabbit mAb [PSH14-86]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723683) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-3 protein (HA211168) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723684, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native IL-3 in WEHI-3 cultured for 3 days cell culture supernatant.<br /><br />Capture: <a href="/products/HA723683" style="font-weight: bold;text-decoration: underline;">HA723683</a>, Mouse IL-3 Rabbit mAb [PSH14-85]<br />Detector: <a href="/products/HA723684" style="font-weight: bold;text-decoration: underline;">HA723684</a>, Mouse IL-3 Rabbit mAb [PSH14-86]<br /><br />The concentrations of IL-3 were measured in triplicates, interpolated from the IL-3 standard curve and corrected for sample dilution. Undiluted samples are WEHI-3 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean IL-3 concentration was determined to be 6,529 pg/ml in WEHI-3 cell culture supernatant.](https://storage.huabio.cn/huabio/productImg/HA723684_2.png?v=20260429162017)
Interpolated concentrations of native IL-3 in WEHI-3 cultured for 3 days cell culture supernatant.
Capture: HA723683, Mouse IL-3 Rabbit mAb [PSH14-85]
Detector: HA723684, Mouse IL-3 Rabbit mAb [PSH14-86]
The concentrations of IL-3 were measured in triplicates, interpolated from the IL-3 standard curve and corrected for sample dilution. Undiluted samples are WEHI-3 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean IL-3 concentration was determined to be 6,529 pg/ml in WEHI-3 cell culture supernatant. -
![Interpolated concentrations of spiked IL-3 in mouse cell culture media samples.<br /><br />Capture: <a href="/products/HA723683" style="font-weight: bold;text-decoration: underline;">HA723683</a>, Mouse IL-3 Rabbit mAb [PSH14-85]<br />Detector: <a href="/products/HA723684" style="font-weight: bold;text-decoration: underline;">HA723684</a>, Mouse IL-3 Rabbit mAb [PSH14-86]<br /><br />The concentrations of IL-3 were measured in duplicates, interpolated from the IL-3 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723684_3.png?v=20260429162017)
Interpolated concentrations of spiked IL-3 in mouse cell culture media samples.
Capture: HA723683, Mouse IL-3 Rabbit mAb [PSH14-85]
Detector: HA723684, Mouse IL-3 Rabbit mAb [PSH14-86]
The concentrations of IL-3 were measured in duplicates, interpolated from the IL-3 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"