Human RBP4 Recombinant Rabbit Monoclonal Antibody [PSH14-88] - BSA and Azide free (Capture)
Overview
Product Name
Human RBP4 Recombinant Rabbit Monoclonal Antibody [PSH14-88] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human RBP4 aa 19-201 (HA210999).
Species Reactivity
Human
Validated Applications
ELISA(Cap)
Positive Control
Recombinant Human RBP4 protein (HA210999).
Conjugation
unconjugated
Clone Number
PSH14-88
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-89] to Human RBP4 antibody (Detector) (HA723689) and Recombinant Human RBP4 protein (HA210999) as the standard. The reference range value is 15.6-2,000 pg/mL.
Target
Function
Retinol binding protein 4, also known as RBP4, is a transporter protein for retinol (vitamin A alcohol). RBP4 has a molecular weight of approximately 21 kDa and is encoded by the RBP4 gene in humans. It is mainly, though not exclusively, synthesized in the liver and circulates in the bloodstream as a hepatokine bound to retinol in a complex with transthyretin. RBP4 has been a drug target for ophthalmology research due to its role in vision. RBP4 may also be involved in metabolic diseases as suggested by recent studies. This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells.
Background References
1. Zhang KZ et al. RBP4 promotes denervation-induced muscle atrophy through STRA6-dependent pathway. J Cachexia Sarcopenia Muscle. 2024 Aug
2. Steinhoff JS et al. Biological Functions of RBP4 and Its Relevance for Human Diseases. Front Physiol. 2021 Mar
Subcellular Location
Secreted.
UNIPROT
Synonyms
OTTHUMP00000020114 antibody
OTTHUMP00000020115 antibody
OTTHUMP00000020116 antibody
Plasma retinol binding protein 4 antibody
Plasma retinol-binding protein antibody
Plasma retinol-binding protein(1-176) antibody
PRBP antibody
PRO2222 antibody
RBP antibody
RBP4 antibody
ExpandImages
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![Sandwich ELISA analysis of Human RBP4 matched pair antibodies<br /><br />Capture: <a href="/products/HA723688" style="font-weight: bold;text-decoration: underline;">HA723688</a>, Human RBP4 Rabbit mAb [PSH14-88]<br />Detector: <a href="/products/HA723689" style="font-weight: bold;text-decoration: underline;">HA723689</a>, Human RBP4 Rabbit mAb [PSH14-89]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723688" style="font-weight: bold;text-decoration: underline;">HA723688</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human RBP4 protein (<a href="/products/HA210999" style="font-weight: bold;text-decoration: underline;">HA210999</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723689" style="font-weight: bold;text-decoration: underline;">HA723689</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723688_1.png?v=20260429162019)
Sandwich ELISA analysis of Human RBP4 matched pair antibodies
Capture: HA723688, Human RBP4 Rabbit mAb [PSH14-88]
Detector: HA723689, Human RBP4 Rabbit mAb [PSH14-89]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723688) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human RBP4 protein (HA210999) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723689, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native RBP4 in human samples.<br /><br />Capture: <a href="/products/HA723688" style="font-weight: bold;text-decoration: underline;">HA723688</a>, Human RBP4 Rabbit mAb [PSH14-88]<br />Detector: <a href="/products/HA723689" style="font-weight: bold;text-decoration: underline;">HA723689</a>, Human RBP4 Rabbit mAb [PSH14-89]<br /><br />Interpolated concentration of native RBP4 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean RBP4 concentration was determined to be 208,320 pg/mL in HepG2 cell supernatant. There was no detectable signal in Hela cell culture supernatant.](https://storage.huabio.cn/huabio/productImg/HA723688_2.png?v=20260429162019)
Interpolated concentrations of native RBP4 in human samples.
Capture: HA723688, Human RBP4 Rabbit mAb [PSH14-88]
Detector: HA723689, Human RBP4 Rabbit mAb [PSH14-89]
Interpolated concentration of native RBP4 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean RBP4 concentration was determined to be 208,320 pg/mL in HepG2 cell supernatant. There was no detectable signal in Hela cell culture supernatant. -
![Interpolated concentrations of spiked RBP4 in cell culture media samples.<br /><br />Capture: <a href="/products/HA723688" style="font-weight: bold;text-decoration: underline;">HA723688</a>, Human RBP4 Rabbit mAb [PSH14-88]<br />Detector: <a href="/products/HA723689" style="font-weight: bold;text-decoration: underline;">HA723689</a>, Human RBP4 Rabbit mAb [PSH14-89]<br /><br />The concentrations of RBP4 were measured in duplicates, interpolated from the RBP4 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723688_3.png?v=20260429162019)
Interpolated concentrations of spiked RBP4 in cell culture media samples.
Capture: HA723688, Human RBP4 Rabbit mAb [PSH14-88]
Detector: HA723689, Human RBP4 Rabbit mAb [PSH14-89]
The concentrations of RBP4 were measured in duplicates, interpolated from the RBP4 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"