Mouse IL-1RA Recombinant Rabbit Monoclonal Antibody [PSH14-98] - BSA and Azide free (Capture)
Overview
Product Name
Mouse IL-1RA Recombinant Rabbit Monoclonal Antibody [PSH14-98] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse IL-1RA aa 27-178 (HA211041).
Species Reactivity
Mouse
Validated Applications
ELISA(Cap)
Positive Control
Recombinant Mouse IL-1RA protein (HA211041).
Conjugation
unconjugated
Clone Number
PSH14-98
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-99] to Mouse IL-1RA antibody (Detector) (HA723698) and Recombinant Mouse IL-1RA protein (HA211041) as the standard. The reference range value is 15.6-4,000 pg/mL.
Target
Function
IL-1RA was initially called the IL-1 inhibitor and was discovered separately in 1984 by two independent laboratories. IL-1RA is an agent that binds non-productively to the cell surface interleukin-1 receptor (IL-1R), the same receptor that binds interleukin 1 family (IL-1), preventing IL-1's from sending a signal to that cell. IL-1RA is a member of the interleukin 1 cytokine family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, and is a natural inhibitor of the pro-inflammatory effect of IL1β. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
Background References
1. Tahtinen S et al. IL-1 and IL-1ra are key regulators of the inflammatory response to RNA vaccines. Nat Immunol. 2022 Apr
2. Yuan SF et al. IL-1RA promotes oral squamous cell carcinoma malignancy through mitochondrial metabolism-mediated EGFR/JNK/SOX2 pathway. J Transl Med. 2023 Jul
Subcellular Location
Secreted, Cytoplasm.
UNIPROT
Synonyms
DIRA antibody
F630041P17Rik antibody
ICIL 1RA antibody
ICIL-1RA antibody
ICIL1RA antibody
IL-1ra antibody
IL-1ra3 antibody
IL-1RN antibody
IL1 inhibitor antibody
IL1F3 antibody
ExpandImages
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![Sandwich ELISA analysis of Mouse IL-1RA matched pair antibodies<br /><br />Capture: <a href="/products/HA723697" style="font-weight: bold;text-decoration: underline;">HA723697</a>, Mouse IL-1RA Rabbit mAb [PSH14-98]<br />Detector: <a href="/products/HA723698" style="font-weight: bold;text-decoration: underline;">HA723698</a>, Mouse IL-1RA Rabbit mAb [PSH14-99]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723697" style="font-weight: bold;text-decoration: underline;">HA723697</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-1RA protein (<a href="/products/HA211041" style="font-weight: bold;text-decoration: underline;">HA211041</a>) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723698" style="font-weight: bold;text-decoration: underline;">HA723698</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723697_1.jpg?v=20260429162021)
Sandwich ELISA analysis of Mouse IL-1RA matched pair antibodies
Capture: HA723697, Mouse IL-1RA Rabbit mAb [PSH14-98]
Detector: HA723698, Mouse IL-1RA Rabbit mAb [PSH14-99]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723697) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-1RA protein (HA211041) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723698, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native c in J774A.1 cell <br />culture supernatant untreated or treated with LPS and IFNγ for 24h.<br /><br />Capture: <a href="/products/HA723697" style="font-weight: bold;text-decoration: underline;">HA723697</a>, Mouse IL-1RA Rabbit mAb [PSH14-98]<br />Detector: <a href="/products/HA723698" style="font-weight: bold;text-decoration: underline;">HA723698</a>, Mouse IL-1RA Rabbit mAb [PSH14-99]<br /><br />Interpolated concentration of native IL-1RA was measured in duplicate at different sample concentrations and interpolated from the IL-1RA standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-1RA concentration was determined to be 131 pg/ml in J774A.1 untreated cell culture supernatant,10634 pg/ml in J774A.1 treated cell culture supernatant.](https://storage.huabio.cn/huabio/productImg/HA723697_2.jpg?v=20260429162021)
Interpolated concentrations of native c in J774A.1 cell
culture supernatant untreated or treated with LPS and IFNγ for 24h.
Capture: HA723697, Mouse IL-1RA Rabbit mAb [PSH14-98]
Detector: HA723698, Mouse IL-1RA Rabbit mAb [PSH14-99]
Interpolated concentration of native IL-1RA was measured in duplicate at different sample concentrations and interpolated from the IL-1RA standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-1RA concentration was determined to be 131 pg/ml in J774A.1 untreated cell culture supernatant,10634 pg/ml in J774A.1 treated cell culture supernatant. -
![Interpolated concentrations of spiked IL-1RA in cell culture media samples.<br /><br />Capture: <a href="/products/HA723697" style="font-weight: bold;text-decoration: underline;">HA723697</a>, Mouse IL-1RA Rabbit mAb [PSH14-98]<br />Detector: <a href="/products/HA723698" style="font-weight: bold;text-decoration: underline;">HA723698</a>, Mouse IL-1RA Rabbit mAb [PSH14-99]<br /><br />The concentrations of IL-1RA were measured in duplicates, interpolated from the IL-1RA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723697_3.jpg?v=20260429162021)
Interpolated concentrations of spiked IL-1RA in cell culture media samples.
Capture: HA723697, Mouse IL-1RA Rabbit mAb [PSH14-98]
Detector: HA723698, Mouse IL-1RA Rabbit mAb [PSH14-99]
The concentrations of IL-1RA were measured in duplicates, interpolated from the IL-1RA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"