MUL1 Recombinant Rabbit Monoclonal Antibody [PSH15-17]
Catalog# HA723721
MUL1 Recombinant Rabbit Monoclonal Antibody [PSH15-17]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
MUL1 Recombinant Rabbit Monoclonal Antibody [PSH15-17]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human MUL1 aa 260-352.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 40 kDa
Positive Control
HEK-293 cell lysate, A431 cell lysate, HeLa cell lysate, RD cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, Mouse thymus tissue lysate, Mouse kidney tissue lysate, Rat thymus tissue lysate, Rat kidney tissue lysate, human kidney tissue.
Conjugation
unconjugated
Clone Number
PSH15-17
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
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| Mouse |
|
|
| Rat |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
IHC-P
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1:50-1:200
Target
Function
Mitochondrial E3 ubiquitin protein ligase 1 (MUL1) is an enzyme that in humans is encoded by the MUL1 gene on chromosome 1. This enzyme localizes to the outer mitochondrial membrane, where it regulates mitochondrial morphology and apoptosis through multiple pathways, including the Akt, JNK, and NF-κB. Its proapoptotic function thus implicates it in cancer and Parkinson's disease. Exhibits weak E3 ubiquitin-protein ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. Can ubiquitinate AKT1 preferentially at 'Lys-284' involving 'Lys-48'-linked polyubiquitination and seems to be involved in regulation of Akt signaling by targeting phosphorylated Akt to proteosomal degradation. Proposed to preferentially act as a SUMO E3 ligase at physiological concentrations. Is anchored in the outer mitochondrial membrane. Plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. The function may implicate its ability to sumoylate DNM1L. Has been observed to shuttle between the mitochondria and peroxisome, where it may also help regulate peroxisome fission. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. Involved in the modulation of innate immune defense against viruses by inhibiting DDX58-dependent antiviral response. Can mediate DDX58 sumoylation and disrupt its polyubiquitination. Can also activate NF-κB to initiate mitochondria-to-nucleus signaling under stress.
Background References
1. Lee MS et al. MUL1-RING recruits the substrate, p53-TAD as a complex with UBE2D2-UB conjugate. FEBS J. 2022 Jun
2. Huang Q et al. Ginsenoside compound K protects against cerebral ischemia/reperfusion injury via Mul1/Mfn2-mediated mitochondrial dynamics and bioenergy. J Ginseng Res. 2023 May
Subcellular Location
Mitochondrion outer membrane, Peroxisome.
Synonyms
0610009K11Rik antibody
AV000801 antibody
C1orf166 antibody
Chromosome 1 open reading frame 166 antibody
E3 SUMO-protein ligase MUL1 antibody
E3 ubiquitin ligase antibody
E3 ubiquitin protein ligase MUL1 antibody
E3 ubiquitin-protein ligase MUL1 antibody
FLJ12875 antibody
GIDE antibody
ExpandImages
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Western blot analysis of MUL1 on different lysates with Rabbit anti-MUL1 antibody (HA723721) at 1/5,000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: A431 cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: RD cell lysate (20 µg/Lane)
Lane 5: RAW264.7 cell lysate (20 µg/Lane)
Lane 6: PC-12 cell lysate (20 µg/Lane)
Lane 7: Mouse thymus tissue lysate (low expression) (40 µg/Lane)
Lane 8: Mouse kidney tissue lysate (40 µg/Lane)
Lane 9: Rat thymus tissue lysate (low expression) (40 µg/Lane)
Lane 10: Rat kidney tissue lysate (40 µg/Lane)
Predicted band size: 40 kDa
Observed band size: 37 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723721) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-MUL1 antibody (HA723721) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723721) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"