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SMC5 Recombinant Rabbit Monoclonal Antibody [PSH15-29]

Usd: 385

Specification

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Catalog# HA723735

SMC5 Recombinant Rabbit Monoclonal Antibody [PSH15-29]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

SMC5 Recombinant Rabbit Monoclonal Antibody [PSH15-29]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human SMC5 aa 901-1,101.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IF-Cell, IHC-P

Molecular Weight

Predicted band size: 129 kDa

Positive Control

HeLa cell lysate, Jurkat cell lysate, U-2 OS cell lysate, HEK-293 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, C6, mouse testis tissue, rat testis tissue.

Conjugation

unconjugated

Clone Number

PSH15-29

Reactivity Data

WB IF-Cell IHC-P
Human
Tested Tested
Not Recommended
Mouse
Tested Tested
Not Recommended
Tested Tested
Rat
Tested Tested
Tested Tested
Tested Tested
Green Monkey
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:200

Target

Function

Structural maintenance of chromosomes protein 5 is a protein encoded by the SMC5 gene in human. The structural maintenance of chromosomes' complex underlying mechanisms involved in the dynamics of chromatin dynamics is unknown, and discoveries are shedding light on the various functions. The SMC complex mediates long-distance interactions that enable higher-order chromatin folding in interphase. The SMC complex has an ATPase activity, a conserved kleisin, and regulatory subunits. SMC protein complexes are involved in DNA repair, transcriptional pathways, regulation of chromosome segregation, and immunity in Arabidopsis. In eukaryotes the structural maintenance chromosomes consists of cohesin (SMC1 AND SMC3), condensin (SMC2 and SMC4), and SMC5/6 complexes.

Background References

1. Solé-Soler R et al. Smc5/6, an atypical SMC complex with two RING-type subunits. Biochem Soc Trans. 2020 Oct

2. Bächer J et al. SMC5/6-Mediated Transcriptional Regulation of Hepatitis B Virus and Its Therapeutic Potential. Viruses. 2024 Oct

Subcellular Location

Nucleus, Chromosome, Nucleus, PML body, telomere.

UNIPROT

SwissProt: Q8IY18 Human

SwissProt: Q8CG46 Mouse

Entrez Gene: 293967 Rat

Synonyms

hSMC 5 antibody

hSMC5 antibody

KIAA0594 antibody

MLZ-453 antibody

OTTHUMP00000063367 antibody

SMC 5 antibody

SMC 5 structural maintenance of chromosomes 5 like 1 antibody

SMC protein 5 antibody

SMC-5 antibody

smc5 antibody

Expand

Images

  • Western blot analysis of SMC5 on different lysates with Rabbit anti-SMC5 antibody (<a href="/products/HA723735" style="font-weight: bold;text-decoration: underline;">HA723735</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: Jurkat cell lysate (20 µg/Lane)<br />Lane 3: U-2 OS cell lysate (20 µg/Lane)<br />Lane 4: HEK-293 cell lysate (20 µg/Lane)<br />Lane 5: COS-1 cell lysate (20 µg/Lane)<br />Lane 6: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 7: C6 cell lysate (20 µg/Lane)<br />Lane 8: Mouse testis tissue lysate (40 µg/Lane)<br />Lane 9: Rat testis tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 129 kDa<br />Observed band size: 129 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723735" style="font-weight: bold;text-decoration: underline;">HA723735</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SMC5 on different lysates with Rabbit anti-SMC5 antibody (HA723735) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: Jurkat cell lysate (20 µg/Lane)
    Lane 3: U-2 OS cell lysate (20 µg/Lane)
    Lane 4: HEK-293 cell lysate (20 µg/Lane)
    Lane 5: COS-1 cell lysate (20 µg/Lane)
    Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 7: C6 cell lysate (20 µg/Lane)
    Lane 8: Mouse testis tissue lysate (40 µg/Lane)
    Lane 9: Rat testis tissue lysate (40 µg/Lane)

    Predicted band size: 129 kDa
    Observed band size: 129 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723735) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of C6 cells labeling SMC5 with Rabbit anti-SMC5 antibody (<a href="/products/HA723735" style="font-weight: bold;text-decoration: underline;">HA723735</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SMC5 antibody (<a href="/products/HA723735" style="font-weight: bold;text-decoration: underline;">HA723735</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of C6 cells labeling SMC5 with Rabbit anti-SMC5 antibody (HA723735) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SMC5 antibody (HA723735) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-SMC5 antibody (<a href="/products/HA723735" style="font-weight: bold;text-decoration: underline;">HA723735</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723735" style="font-weight: bold;text-decoration: underline;">HA723735</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-SMC5 antibody (HA723735) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723735) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-SMC5 antibody (<a href="/products/HA723735" style="font-weight: bold;text-decoration: underline;">HA723735</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723735" style="font-weight: bold;text-decoration: underline;">HA723735</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-SMC5 antibody (HA723735) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723735) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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