Skip to content

Total OXPHOS Antibody Cocktail Recombinant Rabbit Multiclonal Antibody [PSH15-50]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA723756

Total OXPHOS Antibody Cocktail Recombinant Rabbit Multiclonal Antibody [PSH15-50]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Total OXPHOS Antibody Cocktail Recombinant Rabbit Multiclonal Antibody [PSH15-50]

Antibody Type

Recombinant Rabbit multiclonal

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 18/22/29/48/54 kDa

Positive Control

HEK-293 cell lysate, HeLa cell lysate, A549 cell lysate, K-562 cell lysate, MCF7 cell lysate, human breast carcinoma tissue, human liver tissue.

Conjugation

unconjugated

Clone Number

PSH15-50

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200

Target

Background References

暂无

Sequence Similarity

Belongs to the ATPase alpha/beta chains family.

Tissue Specificity

Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.

Post-translational Modification

The N-terminus is blocked.; Acetylated on lysine residues. BLOC1S1 is required for acetylation.

Subcellular Location

Mitochondrion inner membrane.

UNIPROT

SwissProt: O95169 Human

SwissProt: P00403 Human

SwissProt: P21912 Human

SwissProt: P22695 Human

SwissProt: P25705 Human

Synonyms

ASHI antibody

ATP synthase subunit alpha antibody

Atp5a1 antibody

ATP5AL2 antibody

COII antibody

Complex I ASHI subunit antibody

COX 2 antibody

COX II antibody

COX2 antibody

COXII antibody

Expand

Images

  • Western blot analysis of Total OXPHOS Antibody Cocktail on different lysates with Rabbit anti-Total OXPHOS Antibody Cocktail antibody (<a href="/products/HA723756" style="font-weight: bold;text-decoration: underline;">HA723756</a>) at 1/2,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: A549 cell lysate<br />Lane 4: K-562 cell lysate<br />Lane 5: MCF7 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 18/22/29/48/54 kDa<br />Observed band size: 18/22/29/48/54 kDa<br /><br />Exposure time: 15 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723756" style="font-weight: bold;text-decoration: underline;">HA723756</a>) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Total OXPHOS Antibody Cocktail on different lysates with Rabbit anti-Total OXPHOS Antibody Cocktail antibody (HA723756) at 1/2,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: A549 cell lysate
    Lane 4: K-562 cell lysate
    Lane 5: MCF7 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 18/22/29/48/54 kDa
    Observed band size: 18/22/29/48/54 kDa

    Exposure time: 15 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723756) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Total OXPHOS Antibody Cocktail antibody (<a href="/products/HA723756" style="font-weight: bold;text-decoration: underline;">HA723756</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723756" style="font-weight: bold;text-decoration: underline;">HA723756</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Total OXPHOS Antibody Cocktail antibody (HA723756) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723756) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Total OXPHOS Antibody Cocktail antibody (<a href="/products/HA723756" style="font-weight: bold;text-decoration: underline;">HA723756</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723756" style="font-weight: bold;text-decoration: underline;">HA723756</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Total OXPHOS Antibody Cocktail antibody (HA723756) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723756) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation