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Ryanodine receptor 2 / RYR-2 Recombinant Rabbit Monoclonal Antibody [PSH15-54]

Usd: 385

Specification

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Catalog# HA723760

Ryanodine receptor 2 / RYR-2 Recombinant Rabbit Monoclonal Antibody [PSH15-54]

Application

  • WB

  • IHC-P

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Ryanodine receptor 2 / RYR-2 Recombinant Rabbit Monoclonal Antibody [PSH15-54]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human RYR-2 aa 1,301-1,600.

Species Reactivity

Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 565 kDa

Positive Control

Mouse heart tissue lysate, Rat heart tissue lysate, mouse heart tissue.

Conjugation

unconjugated

Clone Number

PSH15-54

Reactivity Data

WB IHC-P
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Not Recommended
Human
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200

Target

Function

Ryanodine receptor 2 (RYR2) is one of a class of ryanodine receptors and a protein found primarily in cardiac muscle. In humans, it is encoded by the RYR2 gene. In the process of cardiac calcium-induced calcium release, RYR2 is the major mediator for sarcoplasmic release of stored calcium ions. The RYR2 protein functions as the major component of a calcium channel located in the sarcoplasmic reticulum that supplies ions to the cardiac muscle during systole. To enable cardiac muscle contraction, calcium influx through voltage-gated L-type calcium channels in the plasma membrane allows calcium ions to bind to RYR2 located on the sarcoplasmic reticulum. This binding causes the release of calcium through RYR2 from the sarcoplasmic reticulum into the cytosol, where it binds to the C domain of troponin, which shifts tropomyosin and allows the myosin ATPase to bind to actin, enabling cardiac muscle contraction. RYR2 channels are associated with many cellular functions, including mitochondrial metabolism, gene expression and cell survival, in addition to their role in cardiomyocyte contraction.

Background References

1. Steinberg C et al. RYR2-ryanodinopathies: from calcium overload to calcium deficiency. Europace. 2023 Jun

2. Fowler ED et al. Molecular, Subcellular, and Arrhythmogenic Mechanisms in Genetic RyR2 Disease. Biomolecules. 2022 Jul

Subcellular Location

Sarcoplasmic reticulum membrane.

UNIPROT

SwissProt: E9Q401 Mouse

SwissProt: B0LPN4 Rat

Synonyms

ARVC2 antibody

ARVD2 antibody

Cardiac muscle ryanodine receptor antibody

Cardiac muscle ryanodine receptor-calcium release channel antibody

hRYR-2 antibody

ryanodine receptor 2 (cardiac) antibody

Ryanodine receptor 2 antibody

RyR antibody

RYR-2 antibody

RyR2 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Ryanodine receptor 2 / RYR-2 on different lysates with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (<a href="/products/HA723760" style="font-weight: bold;text-decoration: underline;">HA723760</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse heart tissue lysate<br />Lane 2: Mouse skeletal muscle tissue lysate (negative)<br />Lane 3: Mouse liver tissue lysate (negative)<br />Lane 4: Rat heart tissue lysate<br />Lane 5: Rat skeletal muscle tissue lysate (negative)<br />Lane 6: Rat liver tissue lysate (negative)<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 565 kDa<br />Observed band size: 565 kDa<br /><br />Exposure time: 1 minute 7 seconds; ECL: K1802;<br /><br />3-8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723760" style="font-weight: bold;text-decoration: underline;">HA723760</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Ryanodine receptor 2 / RYR-2 on different lysates with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (HA723760) at 1/2,000 dilution.

    Lane 1: Mouse heart tissue lysate
    Lane 2: Mouse skeletal muscle tissue lysate (negative)
    Lane 3: Mouse liver tissue lysate (negative)
    Lane 4: Rat heart tissue lysate
    Lane 5: Rat skeletal muscle tissue lysate (negative)
    Lane 6: Rat liver tissue lysate (negative)

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 565 kDa
    Observed band size: 565 kDa

    Exposure time: 1 minute 7 seconds; ECL: K1802;

    3-8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723760) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (<a href="/products/HA723760" style="font-weight: bold;text-decoration: underline;">HA723760</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723760" style="font-weight: bold;text-decoration: underline;">HA723760</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (HA723760) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723760) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (<a href="/products/HA723760" style="font-weight: bold;text-decoration: underline;">HA723760</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723760" style="font-weight: bold;text-decoration: underline;">HA723760</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (HA723760) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723760) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"