Ryanodine receptor 2 / RYR-2 Recombinant Rabbit Monoclonal Antibody [PSH15-54]
Catalog# HA723760
Ryanodine receptor 2 / RYR-2 Recombinant Rabbit Monoclonal Antibody [PSH15-54]
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WB
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IHC-P
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Mouse
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Rat
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unconjugated
Overview
Product Name
Ryanodine receptor 2 / RYR-2 Recombinant Rabbit Monoclonal Antibody [PSH15-54]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human RYR-2 aa 1,301-1,600.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 565 kDa
Positive Control
Mouse heart tissue lysate, Rat heart tissue lysate, mouse heart tissue.
Conjugation
unconjugated
Clone Number
PSH15-54
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | IHC-Fr | |
|---|---|---|---|
| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:200
Target
Function
Ryanodine receptor 2 (RYR2) is one of a class of ryanodine receptors and a protein found primarily in cardiac muscle. In humans, it is encoded by the RYR2 gene. In the process of cardiac calcium-induced calcium release, RYR2 is the major mediator for sarcoplasmic release of stored calcium ions. The RYR2 protein functions as the major component of a calcium channel located in the sarcoplasmic reticulum that supplies ions to the cardiac muscle during systole. To enable cardiac muscle contraction, calcium influx through voltage-gated L-type calcium channels in the plasma membrane allows calcium ions to bind to RYR2 located on the sarcoplasmic reticulum. This binding causes the release of calcium through RYR2 from the sarcoplasmic reticulum into the cytosol, where it binds to the C domain of troponin, which shifts tropomyosin and allows the myosin ATPase to bind to actin, enabling cardiac muscle contraction. RYR2 channels are associated with many cellular functions, including mitochondrial metabolism, gene expression and cell survival, in addition to their role in cardiomyocyte contraction.
Background References
1. Steinberg C et al. RYR2-ryanodinopathies: from calcium overload to calcium deficiency. Europace. 2023 Jun
2. Fowler ED et al. Molecular, Subcellular, and Arrhythmogenic Mechanisms in Genetic RyR2 Disease. Biomolecules. 2022 Jul
Subcellular Location
Sarcoplasmic reticulum membrane.
Synonyms
ARVC2 antibody
ARVD2 antibody
Cardiac muscle ryanodine receptor antibody
Cardiac muscle ryanodine receptor-calcium release channel antibody
hRYR-2 antibody
ryanodine receptor 2 (cardiac) antibody
Ryanodine receptor 2 antibody
RyR antibody
RYR-2 antibody
RyR2 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of Ryanodine receptor 2 / RYR-2 on different lysates with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (HA723760) at 1/2,000 dilution.
Lane 1: Mouse heart tissue lysate
Lane 2: Mouse skeletal muscle tissue lysate (negative)
Lane 3: Mouse liver tissue lysate (negative)
Lane 4: Rat heart tissue lysate
Lane 5: Rat skeletal muscle tissue lysate (negative)
Lane 6: Rat liver tissue lysate (negative)
Lysates/proteins at 40 µg/Lane.
Predicted band size: 565 kDa
Observed band size: 565 kDa
Exposure time: 1 minute 7 seconds; ECL: K1802;
3-8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723760) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (HA723760) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723760) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (HA723760) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723760) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"