Endothelin 1 Recombinant Rabbit Monoclonal Antibody [PSH15-63]
Catalog# HA723770
Endothelin 1 Recombinant Rabbit Monoclonal Antibody [PSH15-63]
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WB
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
Endothelin 1 Recombinant Rabbit Monoclonal Antibody [PSH15-63]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Endothelin 1 aa 1-212.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 24 kDa
Positive Control
HUVEC cell lysate, MDA-MB-231 cell lysate, PC-3M cell lysate, Saos-2 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate.
Conjugation
unconjugated
Clone Number
PSH15-63
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000
Target
Function
Endothelin 1 (ET-1), also known as preproendothelin-1 (PPET1), is a potent vasoconstrictor peptide produced by vascular endothelial cells, as well as by cells in the heart (affecting contractility) and kidney (affecting sodium handling). The protein encoded by this gene – EDN1 – is proteolytically processed to release endothelin 1. Endothelin 1 is one of three isoforms of human endothelin.
Background References
1. Lampsas S et al. Endothelin-1 Plasma and Aqueous Humor Levels in Different Types of Glaucoma: A Systematic Review and Meta-Analysis. Medicina (Kaunas). 2024 Jul
2. Edgerton-Fulton M et al. Endothelin-1 (ET-1) contributes to senescence and phenotypic changes in brain pericytes in diabetes-mimicking conditions. Clin Sci (Lond). 2024 Aug
Subcellular Location
Secreted.
Synonyms
ARCND3 antibody
Big endothelin-1 antibody
EDN1 antibody
EDN1_HUMAN antibody
ET-1 antibody
ET1 antibody
HDLCQ7 antibody
PPET1 antibody
Preproendothelin 1 antibody
Preproendothelin-1 antibody
ExpandImages
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Western blot analysis of Endothelin 1 on different lysates with Rabbit anti-Endothelin 1 antibody (HA723770) at 1/5,000 dilution.
Lane 1: HUVEC cell lysate
Lane 2: MDA-MB-231 cell lysate
Lane 3: PC-3M cell lysate
Lane 4: Saos-2 cell lysate
Lane 5: HeLa cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 24 kDa
Observed band size: 21 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723770) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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