IL-1 alpha Recombinant Rabbit Monoclonal Antibody [PSH15-65]
Catalog# HA723772
IL-1 alpha Recombinant Rabbit Monoclonal Antibody [PSH15-65]
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WB
-
Mouse
-
unconjugated
Overview
Product Name
IL-1 alpha Recombinant Rabbit Monoclonal Antibody [PSH15-65]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse IL-1 alpha aa 115-270.
Species Reactivity
Mouse
Validated Applications
WB
Target Molecular Weight
Predicted band size: 31 kDa
Positive Control
RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate.
Conjugation
unconjugated
Clone Number
PSH15-65
Reactivity Data
| WB | |
|---|---|
| Mouse |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
Target
Function
Interleukin-1 alpha (IL-1 alpha) also known as hematopoietin 1 is a cytokine of the interleukin 1 family that in humans is encoded by the IL1A gene. In general, Interleukin 1 is responsible for the production of inflammation, as well as the promotion of fever and sepsis. IL-1α inhibitors are being developed to interrupt those processes and treat diseases. IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha.
Background References
1. Park MD et al. Hematopoietic aging promotes cancer by fueling IL-1⍺-driven emergency myelopoiesis. Science. 2024 Oct
2. Parlak E et al. Analysis of Chitinase-3-Like Protein 1, IL-1-Alpha, and IL-6 as Novel Inflammatory Biomarkers for COVID-19. J Interferon Cytokine Res. 2022 Oct
Subcellular Location
Nucleus, Cytoplasm, Secreted.
UNIPROT
Synonyms
BAF antibody
FAF antibody
Hematopoietin 1 antibody
Hematopoietin-1 antibody
IL 1 alpha antibody
IL 1A antibody
IL-1 alpha antibody
Il-1a antibody
IL1 ALPHA antibody
IL1 antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of IL-1 alpha on different lysates with Rabbit anti-IL-1 alpha antibody (HA723772) at 1/5,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723772) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"