Mouse M-CSF/CSF1 Recombinant Rabbit Monoclonal Antibody [PSH15-85] - BSA and Azide free (Detector)
Overview
Product Name
Mouse M-CSF/CSF1 Recombinant Rabbit Monoclonal Antibody [PSH15-85] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse M-CSF/CSF1 aa 33-262 (HA211182).
Species Reactivity
Mouse
Validated Applications
ELISA(Det)
Positive Control
Recombinant Mouse M-CSF/CSF1 protein (HA211182).
Conjugation
unconjugated
Clone Number
PSH15-85
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-84] to Mouse M-CSF/CSF1 antibody (Capture) (HA723792) and Recombinant Mouse M-CSF/CSF1 protein (HA211182) as the standard. The reference range value is 7.8-2,000 pg/mL.
Target
Function
The colony stimulating factor 1 (CSF1), also known as macrophage colony-stimulating factor (M-CSF), is a secreted cytokine which causes hematopoietic stem cells to differentiate into macrophages or other related cell types. Eukaryotic cells also produce M-CSF in order to combat intercellular viral infection. It is one of the three experimentally described colony-stimulating factors. M-CSF binds to the colony stimulating factor 1 receptor. It may also be involved in development of the placenta. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy.
Background References
1. Efe G et al. p53 Gain-of-Function Mutation Induces Metastasis via BRD4-Dependent CSF-1 Expression. Cancer Discov. 2023 Dec
2. Chitu V et al. Modeling CSF-1 receptor deficiency diseases - how close are we? FEBS J. 2022 Sep
Subcellular Location
Cell membrane; Secreted, extracellular space.
UNIPROT
Synonyms
Colony stimulating factor 1 (macrophage) antibody
Colony stimulating factor 1 antibody
Colony stimulating factor macrophage specific antibody
CSF 1 antibody
CSF-1 antibody
CSF1 antibody
CSF1_HUMAN antibody
Csfm antibody
Lanimostim antibody
M CSF antibody
ExpandImages
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![Sandwich ELISA analysis of Mouse M-CSF/CSF1 matched pair antibodies<br /><br />Capture: <a href="/products/HA723792" style="font-weight: bold;text-decoration: underline;">HA723792</a>, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84]<br />Detector: <a href="/products/HA723793" style="font-weight: bold;text-decoration: underline;">HA723793</a>, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723792" style="font-weight: bold;text-decoration: underline;">HA723792</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse M-CSF/CSF1 protein (<a href="/products/HA211182" style="font-weight: bold;text-decoration: underline;">HA211182</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723793" style="font-weight: bold;text-decoration: underline;">HA723793</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723793_1.png?v=20260429162043)
Sandwich ELISA analysis of Mouse M-CSF/CSF1 matched pair antibodies
Capture: HA723792, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84]
Detector: HA723793, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723792) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse M-CSF/CSF1 protein (HA211182) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723793, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native M-CSF in mouse LPS and recombinant mouse ING-γ unstimulated and stimulated RAW264.7 cell culture supernatant.<br /><br />Capture: <a href="/products/HA723792" style="font-weight: bold;text-decoration: underline;">HA723792</a>, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84]<br />Detector: <a href="/products/HA723793" style="font-weight: bold;text-decoration: underline;">HA723793</a>, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85]<br /><br />RAW264.7 cells were stimulated with 1 µg/ml LPS and 30 ng/ml recombinant mouse ING-γ or vehicle control and incubated for 24 hours. The concentrations of M-CSF measured in duplicate and interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 731.4 pg/ml in LPS and recombinant mouse ING-γ stimulated RAW264.7 cell culture supernatant and undetectable in the unstimulated RAW264.7 control.](https://storage.huabio.cn/huabio/productImg/HA723793_2.png?v=20260429162043)
Interpolated concentrations of native M-CSF in mouse LPS and recombinant mouse ING-γ unstimulated and stimulated RAW264.7 cell culture supernatant.
Capture: HA723792, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84]
Detector: HA723793, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85]
RAW264.7 cells were stimulated with 1 µg/ml LPS and 30 ng/ml recombinant mouse ING-γ or vehicle control and incubated for 24 hours. The concentrations of M-CSF measured in duplicate and interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 731.4 pg/ml in LPS and recombinant mouse ING-γ stimulated RAW264.7 cell culture supernatant and undetectable in the unstimulated RAW264.7 control. -
![Interpolated concentrations of native M-CSF in mouse LPS and recombinant mouse ING-γ unstimulated and stimulated J774A.1 cell culture supernatant.<br /><br />Capture: <a href="/products/HA723792" style="font-weight: bold;text-decoration: underline;">HA723792</a>, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84]<br />Detector: <a href="/products/HA723793" style="font-weight: bold;text-decoration: underline;">HA723793</a>, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85]<br /><br />J774A.1 cells were stimulated with 1 µg/ml LPS and 100 ng/ml recombinant mouse ING-γ or vehicle control and incubated for 72 hours. The concentrations of M-CSF measured in duplicate and interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 704.2 pg/ml in LPS and recombinant mouse ING-γ stimulated J774A.1 cell culture supernatant and undetectable in the unstimulated J774A.1 control.](https://storage.huabio.cn/huabio/productImg/HA723793_3.png?v=20260429162043)
Interpolated concentrations of native M-CSF in mouse LPS and recombinant mouse ING-γ unstimulated and stimulated J774A.1 cell culture supernatant.
Capture: HA723792, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84]
Detector: HA723793, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85]
J774A.1 cells were stimulated with 1 µg/ml LPS and 100 ng/ml recombinant mouse ING-γ or vehicle control and incubated for 72 hours. The concentrations of M-CSF measured in duplicate and interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 704.2 pg/ml in LPS and recombinant mouse ING-γ stimulated J774A.1 cell culture supernatant and undetectable in the unstimulated J774A.1 control. -
![Interpolated concentrations of spiked M-CSF in mouse cell culture media samples.<br /><br />Capture: <a href="/products/HA723792" style="font-weight: bold;text-decoration: underline;">HA723792</a>, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84]<br />Detector: <a href="/products/HA723793" style="font-weight: bold;text-decoration: underline;">HA723793</a>, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85]<br /><br />The concentrations of M-CSF were measured in triplicates, interpolated from the M-CSF standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3).](https://storage.huabio.cn/huabio/productImg/HA723793_4.png?v=20260429162043)
Interpolated concentrations of spiked M-CSF in mouse cell culture media samples.
Capture: HA723792, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84]
Detector: HA723793, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85]
The concentrations of M-CSF were measured in triplicates, interpolated from the M-CSF standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"