HRP Conjugated Mouse Resistin Recombinant Rabbit Monoclonal Antibody [PSH16-04] - Detector
Overview
Product Name
HRP Conjugated Mouse Resistin Recombinant Rabbit Monoclonal Antibody [PSH16-04] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse Resistin aa 21-114 (HA211042).
Species Reactivity
Mouse
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Mouse Resistin protein (HA211042).
Conjugation
HRP
Clone Number
PSH16-04
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-03] to Mouse Resistin antibody (Capture) (HA723808) and Recombinant Mouse Resistin protein (HA211042) as the standard. The reference range value is 23.4-100,000 pg/mL.
-
ELISA
-
Use at an assay dependent concentration.
Target
Function
Resistin, also known as adipose tissue-specific secretory factor (ADSF) or C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein (XCP1), is a cysteine-rich peptide hormone that is derived from adipose tissue and, in humans, is encoded by the RETN gene. In primates, pigs, and dogs, resistin is secreted primarily by immune and epithelial cells, whereas in rodents, it is mainly secreted by adipose tissue. The human resistin pre-peptide consists of 108 amino acid residues, while in mice and rats it is 114 amino acids in length; the molecular weight is approximately 12.5 kDa. Resistin is classified as an adipose-derived hormone (similar to a cytokine), and its physiological role has been widely debated, particularly regarding its involvement in obesity and type II diabetes mellitus (T2DM).
Background References
1. Tripathi D et al. Resistin in metabolism, inflammation, and disease. FEBS J. 2020 Aug
2. Deb A et al. Resistin: A journey from metabolism to cancer. Transl Oncol. 2021 Oct
Subcellular Location
Secreted.
UNIPROT
Synonyms
Adipose tissue specific secretory factor antibody
Adipose tissue-specific secretory factor antibody
ADSF antibody
C/EBP epsilon regulated myeloid specific secreted cysteine rich protein antibody
C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1 antibody
C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein antibody
CG5403 antibody
Cysteine rich secreted protein A12 alpha like 2 antibody
Cysteine rich secreted protein FIZZ3 antibody
Cysteine-rich secreted protein A12-alpha-like 2 antibody
ExpandImages
-
![Sandwich ELISA analysis of Mouse Resistin matched pair antibodies<br /><br />Capture: <a href="/products/HA723808" style="font-weight: bold;text-decoration: underline;">HA723808</a>, Mouse Resistin Rabbit mAb [PSH16-03]<br />Detector: <a href="/products/HA723809" style="font-weight: bold;text-decoration: underline;">HA723809</a>, Mouse Resistin Rabbit mAb [PSH16-04]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723808" style="font-weight: bold;text-decoration: underline;">HA723808</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Resistin protein (<a href="/products/HA211042" style="font-weight: bold;text-decoration: underline;">HA211042</a>) starting from 100,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723809" style="font-weight: bold;text-decoration: underline;">HA723809</a>, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723809H_1.jpg?v=20260604174734)
Sandwich ELISA analysis of Mouse Resistin matched pair antibodies
Capture: HA723808, Mouse Resistin Rabbit mAb [PSH16-03]
Detector: HA723809, Mouse Resistin Rabbit mAb [PSH16-04]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723808) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Resistin protein (HA211042) starting from 100,000 pg/ml to 0 pg/ml and detect antibody (HA723809, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"