Biotin Conjugated Human CX3CL1 Recombinant Rabbit Monoclonal Antibody [PSH16-09] - Detector
Overview
Product Name
Biotin Conjugated Human CX3CL1 Recombinant Rabbit Monoclonal Antibody [PSH16-09] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CX3CL1 aa 25-341 (HA211057).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human CX3CL1 protein (HA211057).
Conjugation
Biotin
Clone Number
PSH16-09
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-08] to Human CX3CL1 antibody (Capture) (HA723813) and Recombinant Human CX3CL1 protein (HA211057) as the standard. The reference range value is 39.1-10,000 pg/ml.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Fractalkine, also known as chemokine (C-X3-C motif) ligand 1, is a protein that in humans is encoded by the CX3CL1 gene. Fractalkine is found commonly throughout the brain, particularly in neural cells, and its receptor is known to be present on microglial cells. It has also been found to be essential for microglial cell migration. CX3CL1 is also up-regulated in the hippocampus during a brief temporal window following spatial learning, the purpose of which may be to regulate glutamate-mediated neurotransmission tone. This indicates a possible role for the chemokine in the protective plasticity process of synaptic scaling. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22.
Background References
1. Pawelec P et al. The Impact of the CX3CL1/CX3CR1 Axis in Neurological Disorders. Cells. 2020 Oct
2. Subbarayan MS et al. CX3CL1/CX3CR1 signaling targets for the treatment of neurodegenerative diseases. Pharmacol Ther. 2022 Mar
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
A 152E5.2 antibody
AB030188 antibody
ABCD 3 antibody
ABCD3 antibody
AI848747 antibody
C-X3-C motif chemokine 1 antibody
C3Xkine antibody
Chemokine (C-X3-C motif) ligand 1 antibody
Chemokine C X3 C motif ligand 1 antibody
Chemokine CX3C Motif Ligand 1 antibody
ExpandImages
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![Sandwich ELISA analysis of Human CX3CL1 matched pair antibodies<br /><br />Capture: <a href="/products/HA723813" style="font-weight: bold;text-decoration: underline;">HA723813</a>, Human CX3CL1 Rabbit mAb [PSH16-08]<br />Detector: <a href="/products/HA723814" style="font-weight: bold;text-decoration: underline;">HA723814</a>, Human CX3CL1 Rabbit mAb [PSH16-09]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723813" style="font-weight: bold;text-decoration: underline;">HA723813</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CX3CL1protein (<a href="/products/HA211057" style="font-weight: bold;text-decoration: underline;">HA211057</a>) starting from 2000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723814" style="font-weight: bold;text-decoration: underline;">HA723814</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723814B_1.png?v=20260604174751)
Sandwich ELISA analysis of Human CX3CL1 matched pair antibodies
Capture: HA723813, Human CX3CL1 Rabbit mAb [PSH16-08]
Detector: HA723814, Human CX3CL1 Rabbit mAb [PSH16-09]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723813) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CX3CL1protein (HA211057) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA723814, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native CX3CL1 in HUVEC cell <br />culture supernatant untreated or treated with TNFα and IFNγ for 24h.<br /><br />Capture: <a href="/products/HA723813" style="font-weight: bold;text-decoration: underline;">HA723813</a>, Human CX3CL1 Rabbit mAb [PSH16-08]<br />Detector: <a href="/products/HA723814" style="font-weight: bold;text-decoration: underline;">HA723814</a>, Human CX3CL1 Rabbit mAb [PSH16-09]<br /><br />Interpolated concentration of native CX3CL1 was measured in duplicate at different sample concentrations and interpolated from the CX3CL1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL3 concentration was determined to be 9109 pg/ml in HUVEC treated cell culture supernatant, undetectable in HUVEC untreated cell culture supernatant.](https://storage.huabio.cn/huabio/productImg/HA723814B_2.png?v=20260604174751)
Interpolated concentrations of native CX3CL1 in HUVEC cell
culture supernatant untreated or treated with TNFα and IFNγ for 24h.
Capture: HA723813, Human CX3CL1 Rabbit mAb [PSH16-08]
Detector: HA723814, Human CX3CL1 Rabbit mAb [PSH16-09]
Interpolated concentration of native CX3CL1 was measured in duplicate at different sample concentrations and interpolated from the CX3CL1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL3 concentration was determined to be 9109 pg/ml in HUVEC treated cell culture supernatant, undetectable in HUVEC untreated cell culture supernatant. -
![Interpolated concentrations of spiked CX3CL1 in cell culture media samples.<br /><br />Capture: <a href="/products/HA723813" style="font-weight: bold;text-decoration: underline;">HA723813</a>, Human CX3CL1 Rabbit mAb [PSH16-08]<br />Detector: <a href="/products/HA723814" style="font-weight: bold;text-decoration: underline;">HA723814</a>, Human CX3CL1 Rabbit mAb [PSH16-09]<br /><br />The concentrations of CX3CL1 were measured in duplicates, interpolated from the CX3CL1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723814B_3.png?v=20260604174751)
Interpolated concentrations of spiked CX3CL1 in cell culture media samples.
Capture: HA723813, Human CX3CL1 Rabbit mAb [PSH16-08]
Detector: HA723814, Human CX3CL1 Rabbit mAb [PSH16-09]
The concentrations of CX3CL1 were measured in duplicates, interpolated from the CX3CL1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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