Elastin Recombinant Rabbit Monoclonal Antibody [PSH16-13]
Catalog# HA723818
Elastin Recombinant Rabbit Monoclonal Antibody [PSH16-13]
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
Elastin Recombinant Rabbit Monoclonal Antibody [PSH16-13]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Elastin aa 221-270.
Species Reactivity
Human
Validated Applications
IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 68 kDa
Positive Control
Human breast cancer tissue, human lung tissue, human skin tissue, human testis tissue.
Conjugation
unconjugated
Clone Number
PSH16-13
Reactivity Data
| IHC-P | IF-Tissue | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
IHC-P
-
1:500
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IF-Tissue
-
1:100
Target
Function
Elastin is a protein encoded by the ELN gene in humans and several other animals. Elastin is a key component in the extracellular matrix of gnathostomes (jawed vertebrates). It is highly elastic and present in connective tissue of the body to resume its shape after stretching or contracting. Elastin helps skin return to its original position whence poked or pinched. Elastin is also in important load-bearing tissue of vertebrates and used in places where storage of mechanical energy is required. The ELN gene encodes a protein that is one of the two components of elastic fibers. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Multiple transcript variants encoding different isoforms have been found for this gene. Elastin's soluble precursor is tropoelastin.
Background References
1. Lin CJ et al. Elastin, arterial mechanics, and stenosis. Am J Physiol Cell Physiol. 2022 May
2. Gardeazabal L et al. Elastin and collagen fibres in cutaneous wound healing. Exp Dermatol. 2024 Mar
Subcellular Location
Secreted, extracellular space, extracellular matrix.
UNIPROT
Synonyms
Elastin antibody
ELN antibody
ELN_HUMAN antibody
SVAS antibody
Tropoelastin antibody
WBS antibody
WS antibody
Images
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Elastin antibody (HA723818) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723818) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Elastin antibody (HA723818) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723818) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Elastin antibody (HA723818) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723818) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Elastin antibody (HA723818) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723818) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Human
Site: breast cancer
Sample: Paraffin-embedded section
Antibody concentration: 1/100 -
Application: IF-Tissue
Species: Human
Site: lung
Sample: Paraffin-embedded section
Antibody concentration: 1/100 -
Application: IF-Tissue
Species: Human
Site: testis
Sample: Paraffin-embedded section
Antibody concentration: 1/100
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"