Alpha 1 antitrypsin Recombinant Rabbit Monoclonal Antibody [PSH16-21]
Catalog# HA723819
Alpha 1 antitrypsin Recombinant Rabbit Monoclonal Antibody [PSH16-21]
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WB
-
IHC-P
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Mouse
-
Rat
-
unconjugated
Overview
Product Name
Alpha 1 antitrypsin Recombinant Rabbit Monoclonal Antibody [PSH16-21]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse Alpha 1 antitrypsin aa 25-413.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 46 kDa
Positive Control
Mouse liver tissue lysate, Mouse heart tissue lysate, Mouse kidney tissue lysate, Rat spleen ver tissue lysate, mouse liver tissue.
Conjugation
unconjugated
Clone Number
PSH16-21
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Mouse |
|
|
| Rat |
|
|
| Human |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:50,000
-
IHC-P
-
1:10,000
Target
Function
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). A disorder whose most common manifestation is emphysema, which becomes evident by the third to fourth decade. A less common manifestation of the deficiency is liver disease, which occurs in children and adults, and may result in cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age.
Background References
1. Franciosi AN et al. Alpha-1 antitrypsin deficiency-associated panniculitis. J Am Acad Dermatol. 2022 Oct
2. Pires Ferreira D et al. Gene therapy for alpha-1 antitrypsin deficiency: an update. Expert Opin Biol Ther. 2023 Mar
Subcellular Location
Secreted.
Synonyms
A1A antibody
A1AT antibody
A1AT_HUMAN antibody
AAT antibody
Alpha 1 antiproteinase antibody
Alpha 1 antitrypsin antibody
Alpha 1 antitrypsin null antibody
Alpha 1 protease inhibitor antibody
Alpha-1 protease inhibitor antibody
Alpha-1-antiproteinase antibody
ExpandImages
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Western blot analysis of Alpha 1 antitrypsin on different lysates with Rabbit anti-Alpha 1 antitrypsin antibody (HA723819) at 1/50,000 dilution.
Lane 1: Mouse liver tissue lysate (20 µg/Lane)
Lane 2: Mouse heart tissue lysate (20 µg/Lane)
Lane 3: Mouse kidney tissue lysate (20 µg/Lane)
Lane 4: Rat spleen ver tissue lysate (40 µg/Lane)
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723819) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Alpha 1 antitrypsin antibody (HA723819) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723819) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"