Mouse VCAM1 Recombinant Rabbit Monoclonal Antibody [PSH16-31] - BSA and Azide free (Capture)
Overview
Product Name
Mouse VCAM1 Recombinant Rabbit Monoclonal Antibody [PSH16-31] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse VCAM1 aa 25-698 (HA211043).
Species Reactivity
Mouse
Validated Applications
ELISA(Cap)
Positive Control
Recombinant Mouse VCAM1 protein (HA211043).
Conjugation
unconjugated
Clone Number
PSH16-31
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-32] to Mouse VCAM1 antibody (Detector) (HA723830) and Recombinant Mouse VCAM1 protein (HA211043) as the standard. The reference range value is 31.3-8,000 pg/mL.
Target
Function
Vascular cell adhesion protein 1 also known as vascular cell adhesion molecule 1 (VCAM-1) or cluster of differentiation 106 (CD106) is a protein that in humans is encoded by the VCAM1 gene. VCAM-1 functions as a cell adhesion molecule. The VCAM-1 protein mediates the adhesion of lymphocytes, monocytes, eosinophils, and basophils to vascular endothelium. It also functions in leukocyte-endothelial cell signal transduction, and it may play a role in the development of atherosclerosis and rheumatoid arthritis.
Background References
1. Lau SF et al. The VCAM1-ApoE pathway directs microglial chemotaxis and alleviates Alzheimer\'s disease pathology. Nat Aging. 2023 Oct
2. Wang L et al. Endothelial cell-derived extracellular vesicles expressing surface VCAM1 promote sepsis-related acute lung injury by targeting and reprogramming monocytes. J Extracell Vesicles. 2024 Mar
Subcellular Location
Cell membrane; Secreted.
UNIPROT
Synonyms
CD106 antibody
CD106 Antigen antibody
INCAM 100 antibody
INCAM-100 antibody
L1CAM antibody
MGC99561 antibody
V-CAM 1 antibody
Vascular Cell Adhesion Molecule 1 antibody
Vascular cell adhesion protein 1 antibody
VCAM 1 antibody
ExpandImages
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![Sandwich ELISA analysis of Mouse VCAM1 matched pair antibodies<br /><br />Capture: <a href="/products/HA723829" style="font-weight: bold;text-decoration: underline;">HA723829</a>, Mouse VCAM1 Rabbit mAb [PSH16-31]<br />Detector: <a href="/products/HA723830" style="font-weight: bold;text-decoration: underline;">HA723830</a>, Mouse VCAM1 Rabbit mAb [PSH16-32]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723829" style="font-weight: bold;text-decoration: underline;">HA723829</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse VCAM1 protein (<a href="/products/HA211043" style="font-weight: bold;text-decoration: underline;">HA211043</a>) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723830" style="font-weight: bold;text-decoration: underline;">HA723830</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723829_1.png?v=20260429162048)
Sandwich ELISA analysis of Mouse VCAM1 matched pair antibodies
Capture: HA723829, Mouse VCAM1 Rabbit mAb [PSH16-31]
Detector: HA723830, Mouse VCAM1 Rabbit mAb [PSH16-32]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723829) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse VCAM1 protein (HA211043) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723830, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native VCAM1 in Mouse serum and <br />Raw264.7 cell culture supernatantl.<br /><br />Capture: <a href="/products/HA723829" style="font-weight: bold;text-decoration: underline;">HA723829</a>, Mouse VCAM1 Rabbit mAb [PSH16-31]<br />Detector: <a href="/products/HA723830" style="font-weight: bold;text-decoration: underline;">HA723830</a>, Mouse VCAM1 Rabbit mAb [PSH16-32]<br /><br />Interpolated concentration of native VCAM1 was measured in duplicate at different sample concentrations and interpolated from the VCAM1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean VCAM1 concentration was determined to be 3,001,110 pg/ml in Mouse serum and undetectable in Raw264.7 cell culture supernatant.](https://storage.huabio.cn/huabio/productImg/HA723829_2.png?v=20260429162048)
Interpolated concentrations of native VCAM1 in Mouse serum and
Raw264.7 cell culture supernatantl.
Capture: HA723829, Mouse VCAM1 Rabbit mAb [PSH16-31]
Detector: HA723830, Mouse VCAM1 Rabbit mAb [PSH16-32]
Interpolated concentration of native VCAM1 was measured in duplicate at different sample concentrations and interpolated from the VCAM1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean VCAM1 concentration was determined to be 3,001,110 pg/ml in Mouse serum and undetectable in Raw264.7 cell culture supernatant. -
![Interpolated concentrations of spiked VCAM1 in cell culture media samples.<br /><br />Capture: <a href="/products/HA723829" style="font-weight: bold;text-decoration: underline;">HA723829</a>, Mouse VCAM1 Rabbit mAb [PSH16-31]<br />Detector: <a href="/products/HA723830" style="font-weight: bold;text-decoration: underline;">HA723830</a>, Mouse VCAM1 Rabbit mAb [PSH16-32]<br /><br />The concentrations of VCAM1 were measured in duplicates, interpolated from the VCAM1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723829_3.png?v=20260429162048)
Interpolated concentrations of spiked VCAM1 in cell culture media samples.
Capture: HA723829, Mouse VCAM1 Rabbit mAb [PSH16-31]
Detector: HA723830, Mouse VCAM1 Rabbit mAb [PSH16-32]
The concentrations of VCAM1 were measured in duplicates, interpolated from the VCAM1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"