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Neuropeptide Y Recombinant Rabbit Monoclonal Antibody [PSH16-52]

Usd: 385

Specification

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Catalog# HA723856

Neuropeptide Y Recombinant Rabbit Monoclonal Antibody [PSH16-52]

Application

  • IHC-Fr

  • IHC-P

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Neuropeptide Y Recombinant Rabbit Monoclonal Antibody [PSH16-52]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-Fr, IHC-P, WB

Molecular Weight

Predicted band size: 11 kDa

Positive Control

Human cerebrum tissue, mouse cerebrum tissue, rat cerebrum tissue, SH-SY5Y treated with 200nM TPA for 24 hours cell lysate, HEK-293 transfected with Neuropeptide Y then treated with 200nM TPA for 24 hours cell lysate.

Conjugation

unconjugated

Clone Number

PSH16-52

Reactivity Data

IHC-Fr IHC-P WB
Human
Tested Tested
Not Recommended
Mouse
Tested Tested
Tested Tested
Not Recommended
Rat
Tested Tested
Tested Tested
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:1,000

  • WB

  • 1:2,000

Target

Function

Neuropeptide Y (NPY) is a 36-amino acid neuropeptide that is involved in various physiological and homeostatic processes in both the central and peripheral nervous systems. It is secreted alongside other neurotransmitters such as GABA and glutamate. In the autonomic system it is produced mainly by neurons of the sympathetic nervous system and serves as a strong vasoconstrictor and also causes growth of fat tissue. In the brain, it is produced in various locations including the hypothalamus, and is thought to have several functions, including: increasing food intake and storage of energy as fat, reducing anxiety and stress, reducing pain perception, affecting the circadian rhythm, reducing voluntary alcohol intake, lowering blood pressure, and controlling epileptic seizures.

Background References

1. Sánchez ML et al. Neuropeptide Y Peptide Family and Cancer: Antitumor Therapeutic Strategies. Int J Mol Sci. 2023 Jun

2. Chen Z et al. Neuropeptide Y-Mediated Gut Microbiota Alterations Aggravate Postmenopausal Osteoporosis. Adv Sci (Weinh). 2023 Nov

Subcellular Location

Secreted, Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle.

UNIPROT

SwissProt: P01303 Human

SwissProt: P57774 Mouse

SwissProt: P07808 Rat

Synonyms

C-flanking peptide of NPY antibody

CPON antibody

Neuropeptide tyrosine antibody

Neuropeptide Y precursor antibody

NPY antibody

NPY_HUMAN antibody

Pro neuropeptide Y antibody

PYY 4 antibody

PYY4 antibody

Y Neuropeptide antibody

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: brain<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: brain

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: brain<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: brain

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded human cerebrum tissue with Rabbit anti-Neuropeptide Y antibody (<a href="/products/HA723856" style="font-weight: bold;text-decoration: underline;">HA723856</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723856" style="font-weight: bold;text-decoration: underline;">HA723856</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue with Rabbit anti-Neuropeptide Y antibody (HA723856) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723856) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-Neuropeptide Y antibody (<a href="/products/HA723856" style="font-weight: bold;text-decoration: underline;">HA723856</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723856" style="font-weight: bold;text-decoration: underline;">HA723856</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-Neuropeptide Y antibody (HA723856) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723856) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue with Rabbit anti-Neuropeptide Y antibody (<a href="/products/HA723856" style="font-weight: bold;text-decoration: underline;">HA723856</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723856" style="font-weight: bold;text-decoration: underline;">HA723856</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue with Rabbit anti-Neuropeptide Y antibody (HA723856) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723856) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Neuropeptide Y on different lysates with Rabbit anti-Neuropeptide Y antibody (<a href="/products/HA723856" style="font-weight: bold;text-decoration: underline;">HA723856</a>) at 1/2,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate<br />Lane 2: SH-SY5Y treated with 200nM TPA for 24 hours cell lysate<br />Lane 3: HEK-293 transfected with Neuropeptide Y cell lysate<br />Lane 4: HEK-293 transfected with Neuropeptide Y, then treated with 200nM TPA for 24 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 11 kDa<br />Observed band size: 15 kDa<br /><br />Exposure time: Lane 1-2: 59 seconds; Lane 3-4: 10 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723856" style="font-weight: bold;text-decoration: underline;">HA723856</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Neuropeptide Y on different lysates with Rabbit anti-Neuropeptide Y antibody (HA723856) at 1/2,000 dilution.

    Lane 1: SH-SY5Y cell lysate
    Lane 2: SH-SY5Y treated with 200nM TPA for 24 hours cell lysate
    Lane 3: HEK-293 transfected with Neuropeptide Y cell lysate
    Lane 4: HEK-293 transfected with Neuropeptide Y, then treated with 200nM TPA for 24 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 11 kDa
    Observed band size: 15 kDa

    Exposure time: Lane 1-2: 59 seconds; Lane 3-4: 10 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723856) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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