Human RANKL Recombinant Rabbit Monoclonal Antibody [PSH16-59] - BSA and Azide free (Detector)
Overview
Product Name
Human RANKL Recombinant Rabbit Monoclonal Antibody [PSH16-59] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human RANKL aa 140-317 (HA211170).
Species Reactivity
Human
Validated Applications
ELISA(Det)
Positive Control
Recombinant Human RANKL protein (HA211170).
Conjugation
unconjugated
Clone Number
PSH16-59
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-58] to Human RANKL antibody (Capture) (HA723860) or Rabbit monoclonal [PSH16-60] to Human RANKL antibody (Capture) (HA723862) and Recombinant Human RANKL protein (HA211170) as the standard. The reference range value is 15.6-2,000 pg/mL.
Target
Function
Receptor activator of nuclear factor kappa-Β ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is a protein that in humans is encoded by the TNFSF11 gene. RANKL is known as a type II membrane protein and is a member of the tumor necrosis factor (TNF) superfamily. RANKL has been identified to affect the immune system and control bone regeneration and remodeling. RANKL is an apoptosis regulator gene, a binding partner of osteoprotegerin (OPG), a ligand for the receptor RANK and controls cell proliferation by modifying protein levels of Id4, Id2 and cyclin D1. RANKL is expressed in several tissues and organs including: skeletal muscle, thymus, liver, colon, small intestine, adrenal gland, osteoblast, mammary gland epithelial cells, prostate and pancreas. Variation in concentration levels of RANKL throughout several organs reconfirms the importance of RANKL in tissue growth (particularly bone growth) and immune functions within the body.
Background References
1. Li J et al. Artemisinin relieves osteoarthritis by activating mitochondrial autophagy through reducing TNFSF11 expression and inhibiting PI3K/AKT/mTOR signaling in cartilage. Cell Mol Biol Lett. 2022 Jul
2. McDonald MM et al. Osteoclasts recycle via osteomorphs during RANKL-stimulated bone resorption. Cell. 2021 Mar
Subcellular Location
Cell membrane, Cytoplasm, Secreted.
UNIPROT
Synonyms
CD254 antibody
hRANKL2 antibody
ODF antibody
OPGL antibody
OPTB2 antibody
Osteoclast differentiation factor antibody
Osteoprotegerin ligand antibody
Rank Ligand antibody
RANKL antibody
Receptor activator of nuclear factor kappa B ligand antibody
ExpandImages
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![Sandwich ELISA analysis of Human RANKL matched pair antibodies<br /><br />Capture: <a href="/products/HA723860" style="font-weight: bold;text-decoration: underline;">HA723860</a>, Human RANKL Rabbit mAb [PSH16-58]<br />Detector: <a href="/products/HA723861" style="font-weight: bold;text-decoration: underline;">HA723861</a>, Human RANKL Rabbit mAb [PSH16-59]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723860" style="font-weight: bold;text-decoration: underline;">HA723860</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human RANKL protein (<a href="/products/HA211170" style="font-weight: bold;text-decoration: underline;">HA211170</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723861" style="font-weight: bold;text-decoration: underline;">HA723861</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723861_1.png?v=20260429162058)
Sandwich ELISA analysis of Human RANKL matched pair antibodies
Capture: HA723860, Human RANKL Rabbit mAb [PSH16-58]
Detector: HA723861, Human RANKL Rabbit mAb [PSH16-59]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723860) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human RANKL protein (HA211170) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723861, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Sandwich ELISA analysis of Human RANKL matched pair antibodies<br /><br />Capture: <a href="/products/HA723862" style="font-weight: bold;text-decoration: underline;">HA723862</a>, Human RANKL Rabbit mAb [PSH16-60]<br />Detector: <a href="/products/HA723861" style="font-weight: bold;text-decoration: underline;">HA723861</a>, Human RANKL Rabbit mAb [PSH16-59]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723862" style="font-weight: bold;text-decoration: underline;">HA723862</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human RANKL protein (<a href="/products/HA211170" style="font-weight: bold;text-decoration: underline;">HA211170</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723861" style="font-weight: bold;text-decoration: underline;">HA723861</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723861_2.png?v=20260429162058)
Sandwich ELISA analysis of Human RANKL matched pair antibodies
Capture: HA723862, Human RANKL Rabbit mAb [PSH16-60]
Detector: HA723861, Human RANKL Rabbit mAb [PSH16-59]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723862) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human RANKL protein (HA211170) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723861, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of spiked TNFSF11 in human cell culture media samples.<br /><br />Capture: <a href="/products/HA723860" style="font-weight: bold;text-decoration: underline;">HA723860</a>, Human RANKL Rabbit mAb [PSH16-58]<br />Detector: <a href="/products/HA723861" style="font-weight: bold;text-decoration: underline;">HA723861</a>, Human RANKL Rabbit mAb [PSH16-59]<br /><br />The concentrations of TNFSF11 were measured in duplicates, interpolated from the TNFSF11 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723861_3.png?v=20260429162058)
Interpolated concentrations of spiked TNFSF11 in human cell culture media samples.
Capture: HA723860, Human RANKL Rabbit mAb [PSH16-58]
Detector: HA723861, Human RANKL Rabbit mAb [PSH16-59]
The concentrations of TNFSF11 were measured in duplicates, interpolated from the TNFSF11 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). -
![Interpolated concentrations of spiked TNFSF11 in human cell culture media samples.<br /><br />Capture: <a href="/products/HA723862" style="font-weight: bold;text-decoration: underline;">HA723862</a>, Human RANKL Rabbit mAb [PSH16-60]<br />Detector: <a href="/products/HA723861" style="font-weight: bold;text-decoration: underline;">HA723861</a>, Human RANKL Rabbit mAb [PSH16-59]<br /><br />The concentrations of TNFSF11 were measured in duplicates, interpolated from the TNFSF11 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723861_4.png?v=20260429162058)
Interpolated concentrations of spiked TNFSF11 in human cell culture media samples.
Capture: HA723862, Human RANKL Rabbit mAb [PSH16-60]
Detector: HA723861, Human RANKL Rabbit mAb [PSH16-59]
The concentrations of TNFSF11 were measured in duplicates, interpolated from the TNFSF11 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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