ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH16-76]
Catalog# HA723878
ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH16-76]
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WB
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IHC-P
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Mouse
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Rat
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unconjugated
Overview
Product Name
ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH16-76]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within rat ICAM1 aa 1-517.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 60 kDa
Positive Control
C6 cell lysate, Mouse kidney tissue lysate, Mouse spleen tissue lysate, Mouse liver tissue lysate, Rat spleen tissue lysate, Rat kidney tissue lysate, RAW264.7 cell lysate, mouse kidney tissue, mouse lung tissue, rat kidney tissue, rat lung tissue, rat liver tissue.
Conjugation
unconjugated
Clone Number
PSH16-76
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | IHC-Fr | FC | IF-Cell | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000-1:10,000
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IHC-P
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1:1,000
Target
Function
Cell adhesion molecules (CAMs) are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play important, yet separate, roles in embryogenesis and development. The intracellular adhesion molecule-1 (ICAM-1), also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily and recognizes the beta2alpha1 and beta2alphaM Integrins. ICAM-2 functions as a ligand for lymphocyte function-associated antigen-1 (LFA-1) and is involved in leukocyte adhesion. ICAM-3 is highly expressed on the surface of human eosinophils and, when bound to ligand, may inhibit eosinophil inflammatory responses and survival. ICAM-4, also known as LW glycoprotein, interacts with Integrins alphaLbeta2, alphaMbeta2, alpha4beta1, the alphaV family and alphaIIbbeta3, and selective binding to different integrins may be relevant to the pathology in a number of red blood cell associated diseases. Lastly, ICAM-5, expressed on telencephalic neurons, binds CD11a/CD18 and thus may act as an adhesion molecule for leukocyte binding in the central nervous system.
Background References
1. Grover JR et al. Basic motifs target PSGL-1, CD43, and CD44 to plasma membrane sites where HIV-1 assembles. J Virol 89:454-67 (2015).
2. Rouault C et al. Roles of chemokine ligand-2 (CXCL2) and neutrophils in influencing endothelial cell function and inflammation of human adipose tissue. Endocrinology 154:1069-79 (2013).
Subcellular Location
Membrane.
Synonyms
Antigen identified by monoclonal antibody
BB2 antibody
BB 2 antibody
BB2 antibody
CD 54 antibody
CD_antigen=CD54 antibody
CD54 antibody
Cell surface glycoprotein P3.58 antibody
Human rhinovirus receptor antibody
ICAM 1 antibody
ExpandImages
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Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (HA723878) at 1/10,000 dilution.
Lane 1: C6 cell lysate (20 µg/Lane)
Lane 2: Mouse kidney tissue lysate (30 µg/Lane)
Lane 3: Mouse spleen tissue lysate (30 µg/Lane)
Lane 4: Mouse liver tissue lysate (30 µg/Lane)
Lane 5: Rat spleen tissue lysate (30 µg/Lane)
Lane 6: Rat kidney tissue lysate (30 µg/Lane)
Predicted band size: 60 kDa
Observed band size: 75-100 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723878) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (HA723878) at 1/5,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: RAW264.7 cell lysate treated with deglycosylation
Lysates/proteins at 20 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 60-120 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723878) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-ICAM1 antibody (HA723878) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723878) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-ICAM1 antibody (HA723878) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723878) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-ICAM1 antibody (HA723878) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723878) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-ICAM1 antibody (HA723878) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723878) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-ICAM1 antibody (HA723878) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723878) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"