Mouse/Rat SCF Recombinant Rabbit Monoclonal Antibody [PSH16-93] - BSA and Azide free (Capture)
Overview
Product Name
Mouse/Rat SCF Recombinant Rabbit Monoclonal Antibody [PSH16-93] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse SCF aa 26-214 (HA211067).
Species Reactivity
Mouse, Rat
Validated Applications
ELISA(Cap)
Positive Control
Recombinant Mouse SCF protein (HA211067).
Conjugation
unconjugated
Clone Number
PSH16-93
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-92] to Mouse/Rat SCF antibody (Detector) (HA723888) and Recombinant Mouse SCF protein (HA211067) as the standard. The reference range value is 7.8-2,000 pg/mL.
Target
Function
Stem cell factor (SCF) is the ligand for the transmembrane tyrosine kinase receptor proto-oncogene c-Kit. SCF, also designated KL, MGF and SLF, is a pleiotropic cytokine that has two alternatively spliced forms, 248 and 220 amino acids in length in human and mouse systems, respectively. Both the larger form and the smaller form are cleaved to produce soluble forms. The smaller splice variant lacks the proteolytic cleavage site, between amino acids 149 and 177 of the larger SCF form. The larger form of SCF is expressed in fibroblasts, brain and thymus, while the smaller form is found in spleen, testis, placenta and cerebellum. The SCF ligand is essential for the development of germ cells, hematopoietic progenitor cells and melanocyte precursors. SCF can stimulate the proliferation of mature mast cells as well as the maturation and proliferation of immature mast cells.
Background References
1. Xiang FL et al. Cardiac-specific overexpression of human stem cell factor promotes epicardial activation and arteriogenesis after myocardial infarction. Circ Heart Fail 7:831-42 (2014).
2. Nishikori Y et al. The role of mast cells in cutaneous wound healing in streptozotocin-induced diabetic mice. Arch Dermatol Res 306:823-35 (2014).
Subcellular Location
Cell membrane, Cytoplasm, Secreted.
Synonyms
C kit ligand antibody
C-kit ligand antibody
Ckit ligand antibody
DCUA antibody
DFNA69 antibody
DKFZp686F2250 antibody
familial progressive hyperpigmentation 2 antibody
FPH2 antibody
FPHH antibody
KIT ligand antibody
ExpandImages
-
![Sandwich ELISA analysis of Mouse SCF matched pair antibodies<br /><br />Capture: <a href="/products/HA723887" style="font-weight: bold;text-decoration: underline;">HA723887</a>, Mouse/Rat SCF Rabbit mAb [PSH16-91]<br />Detector: <a href="/products/HA723888" style="font-weight: bold;text-decoration: underline;">HA723888</a>, Mouse/Rat SCF Rabbit mAb [PSH16-92]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723887" style="font-weight: bold;text-decoration: underline;">HA723887</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse SCF protein (<a href="/products/HA211067" style="font-weight: bold;text-decoration: underline;">HA211067</a>) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723888" style="font-weight: bold;text-decoration: underline;">HA723888</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723889_1.png?v=20260908001632)
Sandwich ELISA analysis of Mouse SCF matched pair antibodies
Capture: HA723887, Mouse/Rat SCF Rabbit mAb [PSH16-91]
Detector: HA723888, Mouse/Rat SCF Rabbit mAb [PSH16-92]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723887) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse SCF protein (HA211067) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723888, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native SCF in mouse samples.<br /><br />Capture: <a href="/products/HA723887" style="font-weight: bold;text-decoration: underline;">HA723887</a>, Mouse/Rat SCF Rabbit mAb [PSH16-91]<br />Detector: <a href="/products/HA723888" style="font-weight: bold;text-decoration: underline;">HA723888</a>, Mouse/Rat SCF Rabbit mAb [PSH16-92]<br /><br />Interpolated concentration of native SCF was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean SCF concentration was determined to be 4,551 pg/mL in L929 and 367 pg/mL in J774A.1 cell supernatant.](https://storage.huabio.cn/huabio/productImg/HA723889_2.png?v=20260908001632)
Interpolated concentrations of native SCF in mouse samples.
Capture: HA723887, Mouse/Rat SCF Rabbit mAb [PSH16-91]
Detector: HA723888, Mouse/Rat SCF Rabbit mAb [PSH16-92]
Interpolated concentration of native SCF was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean SCF concentration was determined to be 4,551 pg/mL in L929 and 367 pg/mL in J774A.1 cell supernatant. -
![Interpolated concentrations of spiked SCF in cell culture media samples.<br /><br />Capture: <a href="/products/HA723887" style="font-weight: bold;text-decoration: underline;">HA723887</a>, Mouse/Rat SCF Rabbit mAb [PSH16-91]<br />Detector: <a href="/products/HA723888" style="font-weight: bold;text-decoration: underline;">HA723888</a>, Mouse/Rat SCF Rabbit mAb [PSH16-92]<br /><br />The concentrations of SCF were measured in duplicates, interpolated from the SCF standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723889_3.png?v=20260908001632)
Interpolated concentrations of spiked SCF in cell culture media samples.
Capture: HA723887, Mouse/Rat SCF Rabbit mAb [PSH16-91]
Detector: HA723888, Mouse/Rat SCF Rabbit mAb [PSH16-92]
The concentrations of SCF were measured in duplicates, interpolated from the SCF standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"