CD31 Recombinant Antibody [7-A1-R] - Rabbit IgG (Chimeric) (HA723897)
Catalog# HA723897
to CD31
Tested for
-
WB
-
IF-Cell
-
IHC-P
-
FC
-
mIHC
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CD31 on different lysates with Rabbit anti-CD31 antibody (HA723897) at 1/5,000 dilution.
Lane 1: U-937 cell lysate
Lane 2: HeLa cell lysate (negative)
Lane 3: THP-1 cell lysate
Lane 4: MDA-MB-231 cell lysate (negative)
Lane 5: Jurkat cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 83 kDa
Observed band size: 130 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723897) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD31 on different lysates with Rabbit anti-CD31 antibody (HA723897) at 1/5,000 dilution.
Lane 1: U-937 cell lysate
Lane 2: HeLa cell lysate (negative)
Lane 3: THP-1 cell lysate
Lane 4: MDA-MB-231 cell lysate (negative)
Lane 5: Jurkat cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 83 kDa
Observed band size: 130 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723897) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.