Mouse IL-17F Recombinant Rabbit Monoclonal Antibody [PSH17-15] - BSA and Azide free (Capture/Detector)

Sandwich ELISA analysis of Mouse IL-17F matched pair antibodies

Capture: HA723901, Mouse IL-17F Rabbit mAb [PSH17-14]
Detector: HA723902, Mouse IL-17F Rabbit mAb [PSH17-15]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723901) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-17F protein (HA211035) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723902, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Mouse IL-17F matched pair antibodies

Capture: HA723902, Mouse IL-17F Rabbit mAb [PSH17-15]
Detector: HA723903, Mouse IL-17F Rabbit mAb [PSH17-16]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723902) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-17F protein (HA211035) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723903, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of spiked IL-17F in cell culture media samples.

Capture: HA723901, Mouse IL-17F Rabbit mAb [PSH17-14]
Detector: HA723902, Mouse IL-17F Rabbit mAb [PSH17-15]

The concentrations of IL-17F were measured in duplicates, interpolated from the IL-17F standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Interpolated concentrations of spiked IL-17F in cell culture media samples.

Capture: HA723902, Mouse IL-17F Rabbit mAb [PSH17-15]
Detector: HA723903, Mouse IL-17F Rabbit mAb [PSH17-16]

The concentrations of IL-17F were measured in duplicates, interpolated from the IL-17F standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).