Biotin Conjugated Mouse EPO/Erythropoietin Recombinant Rabbit Monoclonal Antibody [PSH17-22] - Detector
Overview
Product Name
Biotin Conjugated Mouse EPO/Erythropoietin Recombinant Rabbit Monoclonal Antibody [PSH17-22] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse EPO aa 27-192 (HA211068).
Species Reactivity
Mouse
Validated Applications
ELISA(Det)
Positive Control
Recombinant Mouse EPO/Erythropoietin protein (HA211068).
Conjugation
Biotin
Clone Number
PSH17-22
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-21] to Mouse EPO/Erythropoietin antibody (Capture) (HA723908) or Rabbit monoclonal [PSH17-23] to Mouse EPO/Erythropoietin antibody (Capture) (HA723910) and Recombinant Mouse EPO/Erythropoietin protein (HA211068) as the standard. The reference range value is 7.8-2,000 pg/mL.
Target
Function
Erythropoietin (EPO), also known as erythropoetin, haematopoietin, or haemopoietin, is a glycoprotein cytokine secreted mainly by the kidneys in response to cellular hypoxia; it stimulates red blood cell production (erythropoiesis) in the bone marrow. Low levels of EPO (around 10 mU/mL) are constantly secreted in sufficient quantities to compensate for normal red blood cell turnover. Common causes of cellular hypoxia resulting in elevated levels of EPO (up to 10 000 mU/mL) include any anemia, and hypoxemia due to chronic lung disease. Erythropoietin is largely synthesized by fibroblast-like type-1 interstitial cells, located primarily in the deep renal cortex in close association with the peritubular capillaries and proximal convoluted tubule; it is also produced in perisinusoidal cells in the liver. Liver production predominates in the fetal and perinatal period; renal production predominates in adulthood. It is homologous with thrombopoietin.
Background References
1. Metallinou C et al. NGF, EPO, and IGF-1 in the Male Reproductive System. J Clin Med. 2024 May
2. Wu G et al. EPO promotes the progression of rheumatoid arthritis by inducing desialylation via increasing the expression of neuraminidase 3. Ann Rheum Dis. 2024 Apr
Subcellular Location
Secreted.
UNIPROT
Synonyms
EP antibody
EPO alpha antibody
EPO antibody
Epoetin antibody
Erythropoetin antibody
Erythropoietin precursor antibody
MVCD2 antibody
Images
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![Sandwich ELISA analysis of Mouse EPO/Erythropoietin matched pair antibodies<br /><br />Capture: <a href="/products/HA723908" style="font-weight: bold;text-decoration: underline;">HA723908</a>, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-21]<br />Detector: <a href="/products/HA723909" style="font-weight: bold;text-decoration: underline;">HA723909</a>, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-22]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723908" style="font-weight: bold;text-decoration: underline;">HA723908</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse EPO/Erythropoietin protein (<a href="/products/HA211068" style="font-weight: bold;text-decoration: underline;">HA211068</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723909" style="font-weight: bold;text-decoration: underline;">HA723909</a>, Biotin, 0.1 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723909B_1.png?v=20260604174732)
Sandwich ELISA analysis of Mouse EPO/Erythropoietin matched pair antibodies
Capture: HA723908, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-21]
Detector: HA723909, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-22]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723908) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse EPO/Erythropoietin protein (HA211068) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723909, Biotin, 0.1 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Sandwich ELISA analysis of Mouse EPO/Erythropoietin matched pair antibodies<br /><br />Capture: <a href="/products/HA723910" style="font-weight: bold;text-decoration: underline;">HA723910</a>, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-23]<br />Detector: <a href="/products/HA723909" style="font-weight: bold;text-decoration: underline;">HA723909</a>, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-22]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723910" style="font-weight: bold;text-decoration: underline;">HA723910</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse EPO/Erythropoietin protein (<a href="/products/HA211068" style="font-weight: bold;text-decoration: underline;">HA211068</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723909" style="font-weight: bold;text-decoration: underline;">HA723909</a>, Biotin, 0.1 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723909B_2.png?v=20260604174732)
Sandwich ELISA analysis of Mouse EPO/Erythropoietin matched pair antibodies
Capture: HA723910, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-23]
Detector: HA723909, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-22]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723910) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse EPO/Erythropoietin protein (HA211068) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723909, Biotin, 0.1 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native Erythropoietin in mouse Balb/C serum samples.<br /><br />Capture: <a href="/products/HA723908" style="font-weight: bold;text-decoration: underline;">HA723908</a>, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-21]<br />Detector: <a href="/products/HA723909" style="font-weight: bold;text-decoration: underline;">HA723909</a>, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-22]<br /><br />The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curve and corrected for sample dilution. Undiluted samples are Balb/C serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 147.9 pg/ml in Balb/C serum.](https://storage.huabio.cn/huabio/productImg/HA723909B_3.png?v=20260604174732)
Interpolated concentrations of native Erythropoietin in mouse Balb/C serum samples.
Capture: HA723908, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-21]
Detector: HA723909, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-22]
The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curve and corrected for sample dilution. Undiluted samples are Balb/C serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 147.9 pg/ml in Balb/C serum. -
![Interpolated concentrations of native Erythropoietin in mouse Balb/C serum samples.<br /><br />Capture: <a href="/products/HA723910" style="font-weight: bold;text-decoration: underline;">HA723910</a>, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-23]<br />Detector: <a href="/products/HA723909" style="font-weight: bold;text-decoration: underline;">HA723909</a>, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-22]<br /><br />The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curve and corrected for sample dilution. Undiluted samples are Balb/C serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 132.7 pg/ml in Balb/C serum.](https://storage.huabio.cn/huabio/productImg/HA723909B_4.png?v=20260604174732)
Interpolated concentrations of native Erythropoietin in mouse Balb/C serum samples.
Capture: HA723910, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-23]
Detector: HA723909, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-22]
The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curve and corrected for sample dilution. Undiluted samples are Balb/C serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 132.7 pg/ml in Balb/C serum. -
![Interpolated concentrations of spiked Erythropoietin in mouse cell culture media samples.<br /><br />Capture: <a href="/products/HA723908" style="font-weight: bold;text-decoration: underline;">HA723908</a>, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-21]<br />Detector: <a href="/products/HA723909" style="font-weight: bold;text-decoration: underline;">HA723909</a>, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-22]<br /><br />The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723909B_5.png?v=20260604174732)
Interpolated concentrations of spiked Erythropoietin in mouse cell culture media samples.
Capture: HA723908, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-21]
Detector: HA723909, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-22]
The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). -
![Interpolated concentrations of spiked Erythropoietin in mouse cell culture media samples.<br /><br />Capture: <a href="/products/HA723910" style="font-weight: bold;text-decoration: underline;">HA723910</a>, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-23]<br />Detector: <a href="/products/HA723909" style="font-weight: bold;text-decoration: underline;">HA723909</a>, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-22]<br /><br />The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723909B_6.png?v=20260604174732)
Interpolated concentrations of spiked Erythropoietin in mouse cell culture media samples.
Capture: HA723910, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-23]
Detector: HA723909, Mouse EPO/Erythropoietin Rabbit mAb [PSH17-22]
The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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