Biotin Conjugated Mouse/Rat E-Cadherin Recombinant Rabbit Monoclonal Antibody [PSH17-25] - Detector
Overview
Product Name
Biotin Conjugated Mouse/Rat E-Cadherin Recombinant Rabbit Monoclonal Antibody [PSH17-25] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse E-Cadherin aa 157-709.
Species Reactivity
Mouse, Rat
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Mouse E-Cadherin protein.
Conjugation
Biotin
Clone Number
PSH17-25
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-24] to Mouse/Rat E-Cadherin antibody (Capture) (HA723911) and Recombinant Mouse E-Cadherin protein as the standard. The reference range value is 39.1-10000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Cadherin-1 or Epithelial cadherin (E-cadherin), is a protein that in humans is encoded by the CDH1 gene (not to be confused with the APC/C activator protein CDH1). Mutations are correlated with gastric, breast, colorectal, thyroid, and ovarian cancers. CDH1 has also been designated as CD324 (cluster of differentiation 324). It is a tumor suppressor gene.
Background References
1. Lee G et al. E-Cadherin Induces Serine Synthesis to Support Progression and Metastasis of Breast Cancer. Cancer Res. 2024 Sep
2. Rubtsova SN et.al. Dual role of E-cadherin in cancer cells. Tissue Barriers. 2022 Oct
Subcellular Location
Cell junction, adherens junction, Cell membrane, Golgi apparatus, trans-Golgi network, Cytoplasm, desmosome.
Synonyms
Arc 1 antibody
Cadherin 1 antibody
cadherin 1 type 1 E-cadherin antibody
Cadherin-1 antibody
Cadherin1 antibody
CAM 120/80 antibody
CD 324 antibody
CD324 antibody
CD324 antigen antibody
cdh1 antibody
ExpandImages
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![Sandwich ELISA analysis of Mouse E-Cadherin matched pair antibodies<br /><br />Capture: <a href="/products/HA723911" style="font-weight: bold;text-decoration: underline;">HA723911</a>, Mouse/Rat E-Cadherin Rabbit mAb [PSH17-24]<br />Detector: <a href="/products/HA723912" style="font-weight: bold;text-decoration: underline;">HA723912</a>, Mouse/Rat E-Cadherin Rabbit mAb [PSH17-25]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723911" style="font-weight: bold;text-decoration: underline;">HA723911</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse E-Cadherin protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723912" style="font-weight: bold;text-decoration: underline;">HA723912</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723912B_1.png?v=20260604174732)
Sandwich ELISA analysis of Mouse E-Cadherin matched pair antibodies
Capture: HA723911, Mouse/Rat E-Cadherin Rabbit mAb [PSH17-24]
Detector: HA723912, Mouse/Rat E-Cadherin Rabbit mAb [PSH17-25]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723911) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse E-Cadherin protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723912, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native E-Cadherin in Mouse, Rat serum <br />and NIH/3T3 supernatant.<br /><br />Capture: <a href="/products/HA723911" style="font-weight: bold;text-decoration: underline;">HA723911</a>, Mouse/Rat E-Cadherin Rabbit mAb [PSH17-24]<br />Detector: <a href="/products/HA723912" style="font-weight: bold;text-decoration: underline;">HA723912</a>, Mouse/Rat E-Cadherin Rabbit mAb [PSH17-25]<br /><br />Interpolated concentration of native E-Cadherin was measured in duplicate at different sample concentrations and interpolated from the E-Cadherin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 960,793 pg/ml in Mouse serum 257,170 pg/ml in Rat serum and undetermined in NIH/3T3 supernatant.](https://storage.huabio.cn/huabio/productImg/HA723912B_2.png?v=20260604174732)
Interpolated concentrations of native E-Cadherin in Mouse, Rat serum
and NIH/3T3 supernatant.
Capture: HA723911, Mouse/Rat E-Cadherin Rabbit mAb [PSH17-24]
Detector: HA723912, Mouse/Rat E-Cadherin Rabbit mAb [PSH17-25]
Interpolated concentration of native E-Cadherin was measured in duplicate at different sample concentrations and interpolated from the E-Cadherin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 960,793 pg/ml in Mouse serum 257,170 pg/ml in Rat serum and undetermined in NIH/3T3 supernatant. -
![Interpolated concentrations of native E-Cadherin in mouse liver homogenate extract samples based on a 1000 µg/ml extract load.<br /><br />Capture: <a href="/products/HA723911" style="font-weight: bold;text-decoration: underline;">HA723911</a>, Mouse/Rat E-Cadherin Rabbit mAb [PSH17-24]<br />Detector: <a href="/products/HA723912" style="font-weight: bold;text-decoration: underline;">HA723912</a>, Mouse/Rat E-Cadherin Rabbit mAb [PSH17-25]<br /><br />Interpolated concentration of native E-Cadherin was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 14,881 pg/mL in mouse liver homogenate extract.](https://storage.huabio.cn/huabio/productImg/HA723912B_3.png?v=20260604174732)
Interpolated concentrations of native E-Cadherin in mouse liver homogenate extract samples based on a 1000 µg/ml extract load.
Capture: HA723911, Mouse/Rat E-Cadherin Rabbit mAb [PSH17-24]
Detector: HA723912, Mouse/Rat E-Cadherin Rabbit mAb [PSH17-25]
Interpolated concentration of native E-Cadherin was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 14,881 pg/mL in mouse liver homogenate extract. -
![Interpolated concentrations of spiked E-Cadherin in cell culture media samples.<br /><br />Capture: <a href="/products/HA723911" style="font-weight: bold;text-decoration: underline;">HA723911</a>, Mouse/Rat E-Cadherin Rabbit mAb [PSH17-24]<br />Detector: <a href="/products/HA723912" style="font-weight: bold;text-decoration: underline;">HA723912</a>, Mouse/Rat E-Cadherin Rabbit mAb [PSH17-25]<br /><br />The concentrations of E-Cadherin were measured in duplicates, interpolated from the E-Cadherin standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723912B_4.png?v=20260604174732)
Interpolated concentrations of spiked E-Cadherin in cell culture media samples.
Capture: HA723911, Mouse/Rat E-Cadherin Rabbit mAb [PSH17-24]
Detector: HA723912, Mouse/Rat E-Cadherin Rabbit mAb [PSH17-25]
The concentrations of E-Cadherin were measured in duplicates, interpolated from the E-Cadherin standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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