Mouse Apolipoprotein B/Apo B Recombinant Rabbit Monoclonal Antibody [PSH17-76] - BSA and Azide free (Detector)
Catalog# HA723945
Mouse Apolipoprotein B/Apo B Recombinant Rabbit Monoclonal Antibody [PSH17-76] - BSA and Azide free (Detector)
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ELISA(Det)
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Mouse
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15.6-4,000 pg/mL
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6.8
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unconjugated
Overview
Product Name
Mouse Apolipoprotein B/Apo B Recombinant Rabbit Monoclonal Antibody [PSH17-76] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse Apolipoprotein B aa 401-810 (HA211327).
Species Reactivity
Mouse
Validated Applications
ELISA(Det)
Positive Control
Recombinant Mouse Apolipoprotein B/Apo B protein (HA211327).
Conjugation
unconjugated
Clone Number
PSH17-76
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-75] to Mouse Apolipoprotein B/Apo B2 antibody (Capture) (HA723944) or Rabbit monoclonal [PSH17-77] to Mouse Apolipoprotein B/Apo B2 antibody (Capture) (HA723946) and Recombinant Mouse Apolipoprotein B/Apo B protein (HA211327) as the standard. The reference range value is 15.6-4,000 pg/mL.
Target
Function
Apolipoprotein B (ApoB) is a protein that in humans is encoded by the APOB gene. Its measurement is commonly used to detect the risk of atherosclerotic cardiovascular disease. The protein occurs in the plasma in two main isoforms, ApoB48 and ApoB100. The first is synthesized exclusively by the small intestine, the second by the liver. ApoB-100 is the largest of the apoB group of proteins, consisting of 4563 amino acids, including a 27-amino acid signal peptide and a 4536-amino acid mature protein. Both isoforms are coded by APOB and by a single mRNA transcript larger than 16 kb. ApoB48 is generated when a stop codon (UAA) at residue 2153 is created by RNA editing. There appears to be a trans-acting tissue-specific splicing gene that determines which isoform is ultimately produced.[citation needed] Alternatively, there is some evidence that a cis-acting element several thousand bp upstream determines which isoform is produced. As a result of the RNA editing, ApoB48 and ApoB100 share a common N-terminal sequence, but ApoB48 lacks ApoB100's C-terminal LDL receptor binding region. ApoB48 is named because it constitutes 48% of the sequence for ApoB100. ApoB48 is a unique protein in chylomicrons from the small intestine. After most of the lipids in the chylomicron have been absorbed, ApoB48 returns to the liver as part of the chylomicron remnant, where it is endocytosed and degraded.
Background References
1. De Oliveira-Gomes D et al. Apolipoprotein B: Bridging the Gap Between Evidence and Clinical Practice. Circulation. 2024 Jul
2. Contois JH et al. Standardization of Apolipoprotein B, LDL-Cholesterol, and Non-HDL-Cholesterol. J Am Heart Assoc. 2023 Aug
Subcellular Location
Cytoplasm, Secreted, Lipid droplet.
UNIPROT
Synonyms
Apo B 100 antibody
Apo B antibody
Apo B-100 antibody
Apo B-48 antibody
Apo B100 antibody
Apo B48 antibody
ApoB 100 antibody
ApoB 48 antibody
APOB antibody
Apolipoprotein B (including Ag(x) antigen) antibody
ExpandImages
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Sandwich ELISA analysis of Mouse Apolipoprotein B/Apo B matched pair antibodies
Capture: HA723944, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-75]
Detector: HA723945, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-76]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723944) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Apolipoprotein B/Apo B protein (HA211327) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723945, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Sandwich ELISA analysis of Mouse Apolipoprotein B/Apo B matched pair antibodies
Capture: HA723946, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-77]
Detector: HA723945, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-76]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723946) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Apolipoprotein B/Apo B protein (HA211327) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723945, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentrations of native Apolipoprotein B in mouse samples.
Capture: HA723944, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-75]
Detector: HA723945, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-76]
Interpolated concentration of native Apolipoprotein B was measured in duplicate at different sample concentrations and interpolated from the Apolipoprotein B standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Apolipoprotein B concentration was determined to be 84.467 ug/mL in Mouse serum. There was no detectable signal in NIH/3T3 cell supernatant. This immunoassay is calibrated against highly purified ApoB from human plasma. -
Interpolated concentrations of native Apolipoprotein B in mouse samples.
Capture: HA723946, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-77]
Detector: HA723945, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-76]
Interpolated concentration of native Apolipoprotein B was measured in duplicate at different sample concentrations and interpolated from the Apolipoprotein B standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Apolipoprotein B concentration was determined to be 78.699 ug/mL in mouse serum. There was no detectable signal in NIH/3T3 cell supernatant.This immunoassay is calibrated against highly purified ApoB from human plasma. -
Interpolated concentrations of spiked Apolipoprotein B in cell culture media samples.
Capture: HA723944, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-75]
Detector: HA723945, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-76]
The concentrations of Apolipoprotein B were measured in duplicates, interpolated from the Apolipoprotein B standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). -
Interpolated concentrations of spiked Apolipoprotein B in cell culture media samples.
Capture: HA723946, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-77]
Detector: HA723945, Mouse Apolipoprotein B/Apo B Rabbit mAb [PSH17-76]
The concentrations of Apolipoprotein B were measured in duplicates, interpolated from the Apolipoprotein B standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"