c-Kit Recombinant Rabbit Monoclonal Antibody [PSH17-96]
Catalog# HA723961
c-Kit Recombinant Rabbit Monoclonal Antibody [PSH17-96]
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WB
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IHC-Fr
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IHC-P
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Mouse
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Rat
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HA751645
不含抗保成分
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unconjugated
Overview
Product Name
c-Kit Recombinant Rabbit Monoclonal Antibody [PSH17-96]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse c-Kit aa 1-527.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IHC-Fr, IHC-P
Molecular Weight
Predicted band size: 109 kDa
Positive Control
ES-D3 cell lysate, Mouse brain tissue lysate, Mouse testis tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate, rat cerebellum tissue, rat colon tissue, rat testis tissue.
Conjugation
unconjugated
Clone Number
PSH17-96
Reactivity Data
| WB | IHC-Fr | IHC-P | |
|---|---|---|---|
| Mouse |
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| Rat |
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|
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| Human |
|
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| Green Monkey |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-Fr
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1:500
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IHC-P
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1:200-1:5,000
Target
Function
Proto-oncogene c-KIT is the gene encoding the receptor tyrosine kinase protein known as tyrosine-protein kinase KIT, CD117 (cluster of differentiation 117) or mast/stem cell growth factor receptor (SCFR). KIT is a cytokine receptor expressed on the surface of hematopoietic stem cells as well as other cell types. Altered forms of this receptor may be associated with some types of cancer. KIT is a receptor tyrosine kinase type III, which binds to stem cell factor, also known as "steel factor" or "c-kit ligand". When this receptor binds to stem cell factor (SCF) it forms a dimer that activates its intrinsic tyrosine kinase activity, that in turn phosphorylates and activates signal transduction molecules that propagate the signal in the cell. After activation, the receptor is ubiquitinated to mark it for transport to a lysosome and eventual destruction. Signaling through KIT plays a role in cell survival, proliferation, and differentiation. For instance, KIT signaling is required for melanocyte survival, and it is also involved in haematopoiesis and gametogenesis.
Background References
1. Gao X et al. Regulation of the hematopoietic stem cell pool by C-Kit-associated trogocytosis. Science. 2024 Aug
2. Xiong Y et al. c-Kit signaling potentiates CAR T cell efficacy in solid tumors by CD28- and IL-2-independent co-stimulation. Nat Cancer. 2023 Jul
Subcellular Location
Cell membrane.
Synonyms
C Kit antibody
c-Kit antibody
c-Kit Ligand antibody
CD117 antibody
Kit antibody
Kit Ligand antibody
KIT oncogene antibody
KIT proto oncogene receptor tyrosine kinase antibody
Mast cell growth factor receptor antibody
Mast/stem cell growth factor receptor Kit antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of c-Kit on different lysates with Rabbit anti-c-Kit antibody (HA723961) at 1/5,000 dilution.
Lane 1: ES-D3 cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (negative) (20 µg/Lane)
Lane 3: Mouse brain tissue lysate (20 µg/Lane)
Lane 4: Mouse testis tissue lysate (20 µg/Lane)
Lane 5: Rat brain tissue lysate (20 µg/Lane)
Lane 6: Rat cerebellum tissue lysate (20 µg/Lane)
Predicted band size: 109 kDa
Observed band size: 140 kDa
Exposure time: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723961) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: IHC-Fr
Species: Rat
Site: cerebellum
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-c-Kit antibody (HA723961) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723961) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-c-Kit antibody (HA723961) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723961) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-c-Kit antibody (HA723961) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723961) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"