Phospho-Axl (Y702) Recombinant Rabbit Monoclonal Antibody [PSH17-98]
Catalog# HA723963
Phospho-Axl (Y702) Recombinant Rabbit Monoclonal Antibody [PSH17-98]
-
WB
-
Human
-
unconjugated
Overview
Product Name
Phospho-Axl (Y702) Recombinant Rabbit Monoclonal Antibody [PSH17-98]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Tyr702 of human Axl.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 98 kDa
Positive Control
NCI-H1299 cell lysate, NCI-H1299 treated with 100ng/mL Gas6 for 10 minutes cell lysate.
Conjugation
unconjugated
Clone Number
PSH17-98
Reactivity Data
| WB | |
|---|---|
| Human |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
Target
Function
Tyrosine-protein kinase receptor UFO is a protein that in human is encoded by the AXL gene. The gene was initially designated as UFO, in allusion to the unidentified function of this protein. However, in the years since its discovery, research into AXL's expression profile and mechanism has made it an increasingly attractive target, especially for cancer therapeutics. In recent years, AXL has emerged as a key facilitator of immune escape and drug-resistance by cancer cells, leading to aggressive and metastatic cancers. AXL is a cell surface receptor tyrosine kinase, part of the TAM family of kinases including TYRO3 and MERTK.
Background References
1. Tanaka M et al. Therapeutic Targeting of the Gas6/Axl Signaling Pathway in Cancer. Int J Mol Sci. 2021 Sep
2. Adam-Artigues A et al. AXL - a new player in resistance to HER2 blockade. Cancer Treat Rev. 2023 Dec
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
Adhesion related kinase antibody
AI323647 antibody
Ark antibody
Axl antibody
AXL oncogene antibody
AXL receptor tyrosine kinase antibody
AXL transforming gene antibody
AXL transforming sequence/gene antibody
EC 2.7.10.1 antibody
JTK11 antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-Axl (Y702) on different lysates with Rabbit anti-Phospho-Axl (Y702) antibody (HA723963) at 1/5,000 dilution.
Lane 1: NCI-H1299 cell lysate
Lane 2: NCI-H1299 treated with 100ng/mL Gas6 for 10 minutes cell lysate
Lane 3: NCI-H1299 treated with 100ng/mL Gas6 for 10 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 98 kDa
Observed band size: 140 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723963) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"