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5 Lipoxygenase Recombinant Rabbit Monoclonal Antibody [PSH18-05]

Usd: 385

Specification

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Catalog# HA723968

5 Lipoxygenase Recombinant Rabbit Monoclonal Antibody [PSH18-05]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

5 Lipoxygenase Recombinant Rabbit Monoclonal Antibody [PSH18-05]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human 5 Lipoxygenase aa 151-200.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 78 kDa

Positive Control

HL-60 cell lysate, K-562 cell lysate, Rat placenta tissue lysate, Daudi cell lysate, Mouse placenta tissue lysate.

Conjugation

unconjugated

Clone Number

PSH18-05

Reactivity Data

WB
Human
Tested Tested
Mouse
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

Arachidonate 5-lipoxygenase, also known as ALOX5, 5-lipoxygenase, 5-LOX, or 5-LO, is a non-heme iron-containing enzyme (EC 1.13.11.34) that in humans is encoded by the ALOX5 gene. Arachidonate 5-lipoxygenase is a member of the lipoxygenase family of enzymes. It transforms essential fatty acids (EFA) substrates into leukotrienes as well as a wide range of other biologically active products. ALOX5 is a current target for pharmaceutical intervention in a number of diseases.

Background References

1. Yan M et al. 5-Lipoxygenase as an emerging target against age-related brain disorders. Ageing Res Rev. 2021 Aug

2. Xie S et al. Diminished arachidonate 5-lipoxygenase perturbs phase separation and transcriptional response of Runx2 to reverse pathological ventricular remodeling. EBioMedicine. 2022 Dec

Subcellular Location

Cytoplasm, Nucleus matrix, Nucleus membrane, Cytoplasm, perinuclear region, cytosol, Nucleus envelope, Nucleus intermembrane space.

UNIPROT

SwissProt: P09917 Human

SwissProt: P48999 Mouse

SwissProt: P12527 Rat

Synonyms

5 Lipoxygenase antibody

5 LO antibody

5 LOX antibody

5-lipoxygenase antibody

5-LO antibody

5-LOX antibody

5LOX antibody

5LPG antibody

ALOX 5 antibody

Alox5 antibody

Expand

Images

  • Western blot analysis of 5 Lipoxygenase on different lysates with Rabbit anti-5 Lipoxygenase antibody (<a href="/products/HA723968" style="font-weight: bold;text-decoration: underline;">HA723968</a>) at 1/5,000 dilution.<br /><br />Lane 1: HL-60 cell lysate (20 µg/Lane)<br />Lane 2: K-562 cell lysate (20 µg/Lane)<br />Lane 3: Rat placenta tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 78 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723968" style="font-weight: bold;text-decoration: underline;">HA723968</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of 5 Lipoxygenase on different lysates with Rabbit anti-5 Lipoxygenase antibody (HA723968) at 1/5,000 dilution.

    Lane 1: HL-60 cell lysate (20 µg/Lane)
    Lane 2: K-562 cell lysate (20 µg/Lane)
    Lane 3: Rat placenta tissue lysate (30 µg/Lane)

    Predicted band size: 78 kDa
    Observed band size: 75 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723968) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of 5 Lipoxygenase on different lysates with Rabbit anti-5 Lipoxygenase antibody (<a href="/products/HA723968" style="font-weight: bold;text-decoration: underline;">HA723968</a>) at 1/5,000 dilution.<br /><br />Lane 1: Daudi cell lysate (20 µg/Lane)<br />Lane 2: Mouse placenta tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 78 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 1 minute 50 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723968" style="font-weight: bold;text-decoration: underline;">HA723968</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of 5 Lipoxygenase on different lysates with Rabbit anti-5 Lipoxygenase antibody (HA723968) at 1/5,000 dilution.

    Lane 1: Daudi cell lysate (20 µg/Lane)
    Lane 2: Mouse placenta tissue lysate (30 µg/Lane)

    Predicted band size: 78 kDa
    Observed band size: 75 kDa

    Exposure time: 1 minute 50 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723968) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"