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Complement C3 Recombinant Rabbit Monoclonal Antibody [PSH18-31]

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Specification

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Catalog# HA723984

Complement C3 Recombinant Rabbit Monoclonal Antibody [PSH18-31]

Application

  • WB

  • IHC-Fr

  • IHC-P

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Complement C3 Recombinant Rabbit Monoclonal Antibody [PSH18-31]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse Complement C3 aa 1-1,663.

Species Reactivity

Mouse, Rat

Validated Applications

WB, IHC-Fr, IHC-P

Molecular Weight

Predicted band size: 186 kDa

Positive Control

Mouse liver tissue lysate, Mouse spleen tissue lysate, Rat liver tissue lysate, Rat spleen tissue lysate, mouse liver tissue.

Conjugation

unconjugated

Clone Number

PSH18-31

Reactivity Data

WB IHC-Fr IHC-P
Mouse
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:1,000

Target

Function

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.

Background References

1. Hao W et al. Gut dysbiosis induces the development of depression-like behavior through abnormal synapse pruning in microglia-mediated by complement C3. Microbiome. 2024 Feb

2. Xu L et al. Inhibition of complement C3 signaling ameliorates locomotor and visual dysfunction in autoimmune inflammatory diseases. Mol Ther. 2023 Sep

Subcellular Location

Secreted.

UNIPROT

SwissProt: P01027 Mouse

SwissProt: P01026 Rat

Synonyms

Acylation stimulating protein cleavage product antibody

AHUS5 antibody

ARMD9 antibody

ASP antibody

C3 and PZP like alpha 2 macroglobulin domain containing protein 1 antibody

C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody

C3 antibody

CO3_HUMAN antibody

Complement C3 antibody

Complement C3c alpha' chain fragment 2 antibody

Expand

Images

  • Western blot analysis of Complement C3 on different lysates with Rabbit anti-Complement C3 antibody (<a href="/products/HA723984" style="font-weight: bold;text-decoration: underline;">HA723984</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse liver tissue lysate<br />Lane 2: Mouse spleen tissue lysate<br />Lane 3: Rat liver tissue lysate<br />Lane 4: Rat spleen tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 186 kDa<br />Observed band size: 43-186 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723984" style="font-weight: bold;text-decoration: underline;">HA723984</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Complement C3 on different lysates with Rabbit anti-Complement C3 antibody (HA723984) at 1/2,000 dilution.

    Lane 1: Mouse liver tissue lysate
    Lane 2: Mouse spleen tissue lysate
    Lane 3: Rat liver tissue lysate
    Lane 4: Rat spleen tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 186 kDa
    Observed band size: 43-186 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723984) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: liver<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: liver

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: liver<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: liver

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Complement C3 antibody (<a href="/products/HA723984" style="font-weight: bold;text-decoration: underline;">HA723984</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723984" style="font-weight: bold;text-decoration: underline;">HA723984</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Complement C3 antibody (HA723984) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723984) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"