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MuERVL-Gag Recombinant Rabbit Monoclonal Antibody [PSH19-34]

Usd: 385

Specification

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Catalog# HA724066

MuERVL-Gag Recombinant Rabbit Monoclonal Antibody [PSH19-34]

Application

  • WB

Reactivity

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

MuERVL-Gag Recombinant Rabbit Monoclonal Antibody [PSH19-34]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse Gag aa 201-581.

Species Reactivity

Mouse

Validated Applications

WB

Target Molecular Weight

Predicted band size: 67 kDa

Positive Control

ES-D3 cell lysate, ES-W4 cell lysate.

Conjugation

unconjugated

Clone Number

PSH19-34

Reactivity Data

WB
Mouse
Tested Tested

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

A large number of retrotransposons are expressed when the zygotic genome is first transcribed, including the endogenous retroviruses (ERVs), LINE-1 elements, and the non-autonomous SINE elements. At the 2C stage, MuERV-L/MERVL retrovirus-like elements are transiently de-repressed and produce 3% of the transcribed mRNAs. Following the 2C stage, MERVL-retroelement expression is silenced. The newly study discovered that this regulated pattern of MERVL expression overlapped with greater than one hundred 2C-specific genes that have co-opted regulatory elements from these foreign retroviruses to initiate their transcription.

Background References

暂无

UNIPROT

SwissProt: V9H130 Mouse

Images

  • Western blot analysis of MuERVL-Gag on different lysates with Rabbit anti-MuERVL-Gag antibody (<a href="/products/HA724066" style="font-weight: bold;text-decoration: underline;">HA724066</a>) at 1/5,000 dilution.<br /><br />Lane 1: ES-D3 cell lysate<br />Lane 2: ES-W4 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 67 kDa<br />Observed band size: 67 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA724066" style="font-weight: bold;text-decoration: underline;">HA724066</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MuERVL-Gag on different lysates with Rabbit anti-MuERVL-Gag antibody (HA724066) at 1/5,000 dilution.

    Lane 1: ES-D3 cell lysate
    Lane 2: ES-W4 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 67 kDa
    Observed band size: 67 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724066) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"