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DUX4 Recombinant Rabbit Monoclonal Antibody [PSH20-66]

Usd: 385

Specification

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Catalog# HA724165

DUX4 Recombinant Rabbit Monoclonal Antibody [PSH20-66]

Application

  • WB

Reactivity

  • Human

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

DUX4 Recombinant Rabbit Monoclonal Antibody [PSH20-66]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Species Reactivity

Human, Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 45 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, Mouse testis tissue lysate.

Conjugation

unconjugated

Clone Number

PSH20-66

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Double homeobox, 4 also known as DUX4 is a protein which in humans is encoded by the DUX4 gene. Its misexpression is the cause of facioscapulohumeral muscular dystrophy (FSHD). The DUX4 protein is a transcriptional activator of many genes, one example being paired-like homeodomain transcription factor 1 (PITX1). It likely stimulates zygotic genome activation. The two homeodomains allow DUX4 protein to bind to DNA. The C-terminal domain is involved in target gene activation. DUX is normally expressed in the testes, thymus, and cleavage-stage embryos.

Background References

1. Mocciaro E et al. DUX4 Role in Normal Physiology and in FSHD Muscular Dystrophy. Cells. 2021 Nov

2. Bakewell J et al. DUX4-rearranged B-ALL: deciphering a biological and clinical conundrum. Leukemia. 2025 Dec

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: Q9UBX2 Human

Synonyms

Double homeobox 4 antibody

Double homeobox protein 10 antibody

Double homeobox protein 4 antibody

Double homeobox protein 4/10 antibody

DUX10 antibody

Images

  • Western blot analysis of DUX4 on different lysates with Rabbit anti-DUX4 antibody (<a href="/products/HA724165" style="font-weight: bold;text-decoration: underline;">HA724165</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: HepG2 cell lysate (20 µg/Lane)<br />Lane 3: Mouse testis tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 45 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA724165" style="font-weight: bold;text-decoration: underline;">HA724165</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of DUX4 on different lysates with Rabbit anti-DUX4 antibody (HA724165) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: HepG2 cell lysate (20 µg/Lane)
    Lane 3: Mouse testis tissue lysate (40 µg/Lane)

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724165) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"