Human Interferon alpha 2 Recombinant Rabbit Monoclonal Antibody [PSH21-61] - BSA and Azide free (Detector)

Sandwich ELISA analysis of Human Interferon alpha 2 matched pair antibodies

Capture: HA724227, Human Interferon alpha 2 Rabbit mAb [PSH21-60]
Detector: HA724228, Human Interferon alpha 2 Rabbit mAb [PSH21-61]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724227) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Interferon alpha 2 protein (HA210644) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA724228, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of spiked IFN alpha-2 in cell culture media samples.

Capture: HA724227, Human Interferon alpha 2 Rabbit mAb [PSH21-60]
Detector: HA724228, Human Interferon alpha 2 Rabbit mAb [PSH21-61]

The concentrations of IFN alpha-2 were measured in duplicates, interpolated from the IFN alpha-2 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).