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PCSK9 Recombinant Rabbit Monoclonal Antibody [PSH21-65]

Usd: 385

Specification

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Catalog# HA724232

PCSK9 Recombinant Rabbit Monoclonal Antibody [PSH21-65]

Application

  • WB

  • FC

  • IP

Reactivity

  • Human

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

PCSK9 Recombinant Rabbit Monoclonal Antibody [PSH21-65]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse PCSK9 aa 1-694.

Species Reactivity

Human, Mouse

Validated Applications

WB, FC, IP

Target Molecular Weight

Predicted band size: 74 kDa

Positive Control

Huh7 cell lysate, HeLa cell lysate, BxPC-3 cell lysate, A431 cell lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Mouse colon tissue lysate, Huh7.

Conjugation

unconjugated

Clone Number

PSH21-65

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • FC

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is an enzyme encoded by the PCSK9 gene in humans on chromosome 1. It is the 9th member of the proprotein convertase family of proteins that activate other proteins. Similar genes (orthologs) are found across many species. As with many proteins, PCSK9 is inactive when first synthesized, because a section of peptide chains blocks their activity; proprotein convertases remove that section to activate the enzyme. The PCSK9 gene also contains one of 27 loci associated with increased risk of coronary artery disease.

Background References

1. Hummelgaard S et al. Targeting PCSK9 to tackle cardiovascular disease. Pharmacol Ther. 2023 Sep

2. Seidah NG et al. The Multifaceted Biology of PCSK9. Endocr Rev. 2022 May

Subcellular Location

Cytoplasm, Secreted, Endosome, Lysosome, Cell surface, Endoplasmic reticulum, Golgi apparatus.

UNIPROT

SwissProt: Q8NBP7 Human

SwissProt: Q80W65 Mouse

Synonyms

Convertase subtilisin/kexin type 9 preproprotein antibody

FH3 antibody

HCHOLA3 antibody

Hypercholesterolemia autosomal dominant 3 antibody

LDLCQ1 antibody

NARC 1 antibody

NARC-1 antibody

NARC1 antibody

Neural apoptosis regulated convertase 1 antibody

Neural apoptosis-regulated convertase 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of PCSK9 on different lysates with Rabbit anti-PCSK9 antibody (<a href="/products/HA724232" style="font-weight: bold;text-decoration: underline;">HA724232</a>) at 1/5,000 dilution.<br /><br />Lane 1: Huh7 cell lysate<br />Lane 2: SH-SY5Y cell lysate (negative)<br />Lane 3: HeLa cell lysate<br />Lane 4: BxPC-3 cell lysate<br />Lane 6: A431 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 74 kDa<br />Observed band size: 62/74 kDa<br /><br />Exposure time: 15 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA724232" style="font-weight: bold;text-decoration: underline;">HA724232</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of PCSK9 on different lysates with Rabbit anti-PCSK9 antibody (HA724232) at 1/5,000 dilution.

    Lane 1: Huh7 cell lysate
    Lane 2: SH-SY5Y cell lysate (negative)
    Lane 3: HeLa cell lysate
    Lane 4: BxPC-3 cell lysate
    Lane 6: A431 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 74 kDa
    Observed band size: 62/74 kDa

    Exposure time: 15 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724232) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of PCSK9 on different lysates with Rabbit anti-PCSK9 antibody (<a href="/products/HA724232" style="font-weight: bold;text-decoration: underline;">HA724232</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse liver tissue lysate<br />Lane 2: Mouse kidney tissue lysate<br />Lane 3: Mouse colon tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 74 kDa<br />Observed band size: 62/74 kDa<br /><br />Exposure time: 15 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA724232" style="font-weight: bold;text-decoration: underline;">HA724232</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PCSK9 on different lysates with Rabbit anti-PCSK9 antibody (HA724232) at 1/5,000 dilution.

    Lane 1: Mouse liver tissue lysate
    Lane 2: Mouse kidney tissue lysate
    Lane 3: Mouse colon tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 74 kDa
    Observed band size: 62/74 kDa

    Exposure time: 15 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724232) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Flow cytometric analysis of SH-SY5Y (left, negative) and Huh7 (right, positive) cells labeling PCSK9.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA724232" style="font-weight: bold;text-decoration: underline;">HA724232</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    ☑ Relative expression (RE)

    Flow cytometric analysis of SH-SY5Y (left, negative) and Huh7 (right, positive) cells labeling PCSK9.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA724232, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • PCSK9 was immunoprecipitated from 0.2 mg Huh7 cell lysate with <a href="/products/HA724232" style="font-weight: bold;text-decoration: underline;">HA724232</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA724232" style="font-weight: bold;text-decoration: underline;">HA724232</a> at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: Huh7 cell lysate (input)<br />Lane 2: <a href="/products/HA724232" style="font-weight: bold;text-decoration: underline;">HA724232</a> IP in Huh7 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA724232" style="font-weight: bold;text-decoration: underline;">HA724232</a> in Huh7 cell lysate<br /><br />Blocking/Dilution buffer: primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>)<br />Exposure time: 19 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    PCSK9 was immunoprecipitated from 0.2 mg Huh7 cell lysate with HA724232 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724232 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: Huh7 cell lysate (input)
    Lane 2: HA724232 IP in Huh7 cell lysate
    Lane 3: Rabbit IgG instead of HA724232 in Huh7 cell lysate

    Blocking/Dilution buffer: primary antibody dilution (K1803)
    Exposure time: 19 seconds; ECL: K1801

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