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HRP Conjugated beta Actin Recombinant Rabbit Monoclonal Antibody [PSH03-63] - Loading control

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Catalog# HA724235H

HRP Conjugated beta Actin Recombinant Rabbit Monoclonal Antibody [PSH03-63] - Loading control

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

  • Pig

Conjugation

  • HRP

Overview

Product Name

HRP Conjugated beta Actin Recombinant Rabbit Monoclonal Antibody [PSH03-63] - Loading control

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within N-terminal residues of β-Actin.

Species Reactivity

Human, Mouse, Rat, Green monkey, Pig

Validated Applications

WB

Molecular Weight

Predicted band size: 42 kDa

Positive Control

Mouse brain tissue lysate, Mouse liver tissue lysate, Rat brain tissue lysate, Rat liver tissue lysate, Pig brain tissue lysate, HeLa cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, COS-1 cell lysate.

Conjugation

HRP

Clone Number

PSH03-63

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

Target

Function

Actins are highly conserved proteins involved in cell motility, structure and integrity. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Beta actin is usually used as a loading control, for among others, the integrity of cells, protein degradation, in Western Blotting.

Background References

1. Ponte P., Ng S.Y., Engel J., Gunning P., Kedes L."Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA." Nucleic Acids Res. 12:1687-1696(1984) Ohmori H., Toyama S., Toyama S."Direct proof that the primary site of action of cytochalasin on cell motility processes is actin."J. Cell Biol. 116:933-941(1992)

Subcellular Location

Cytoskeleton.

UNIPROT

SwissProt: P60709 Human

SwissProt: P60710 Mouse

SwissProt: P60711 Rat

Synonyms

A26C1A antibody

A26C1B antibody

ACTB antibody

ACTB_HUMAN antibody

Actin beta antibody

Actin cytoplasmic 1 antibody

Actin, cytoplasmic 1, N-terminally processed antibody

Actx antibody

b actin antibody

Beta cytoskeletal actin antibody

Expand

Images

  • Western blot analysis of beta Actin on different lysates with Rabbit anti-beta Actin antibody (<a href="/products/HA724235H" style="font-weight: bold;text-decoration: underline;">HA724235H</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Mouse liver tissue lysate<br />Lane 3: Rat brain tissue lysate<br />Lane 4: Rat liver tissue lysate<br />Lane 4: Pig brain tissue lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 14 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA724235H" style="font-weight: bold;text-decoration: underline;">HA724235H</a>, HRP) at 1/2,000 dilution was used in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at room temperature for 1 hour.

    Western blot analysis of beta Actin on different lysates with Rabbit anti-beta Actin antibody (HA724235H) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Mouse liver tissue lysate
    Lane 3: Rat brain tissue lysate
    Lane 4: Rat liver tissue lysate
    Lane 4: Pig brain tissue lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 14 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724235H, HRP) at 1/2,000 dilution was used in primary antibody dilution buffer (K1803) at room temperature for 1 hour.

  • Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (<a href="/products/HA724235H" style="font-weight: bold;text-decoration: underline;">HA724235H</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HEK-293 cell lysate<br />Lane 3: NIH/3T3 cell lysate<br />Lane 4: RAW264.7 cell lysate<br />Lane 5: C6 cell lysate<br />Lane 6: PC-12 cell lysate<br />Lane 7: COS-1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA724235H" style="font-weight: bold;text-decoration: underline;">HA724235H</a>, HRP) at 1/2,000 dilution was used in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at room temperature for 1 hour.

    Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (HA724235H) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HEK-293 cell lysate
    Lane 3: NIH/3T3 cell lysate
    Lane 4: RAW264.7 cell lysate
    Lane 5: C6 cell lysate
    Lane 6: PC-12 cell lysate
    Lane 7: COS-1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724235H, HRP) at 1/2,000 dilution was used in primary antibody dilution buffer (K1803) at room temperature for 1 hour.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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