HRP Conjugated Human/Monkey VEGFR3 / FLT4 Recombinant Rabbit Monoclonal Antibody [PSH22-17] - Detector

Sandwich ELISA analysis of Human VEGFR3 matched pair antibodies

Capture: HA724277, Human/Monkey VEGFR3 / FLT4 Rabbit mAb [PSH22-16]
Detector: HA724278, Human/Monkey VEGFR3 / FLT4 Rabbit mAb [PSH22-17]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724277) diluted in carbonate/bicarbonate buffer, at a concentHumanion of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.2% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGFR3 / FLT4 protein (HA211107) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA724278, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB SubstHumane for 10 minutes at room tempeHumanure in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.