P2X7 Recombinant Rabbit Monoclonal Antibody [PSH22-44]
Catalog# HA724296
P2X7 Recombinant Rabbit Monoclonal Antibody [PSH22-44]
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WB
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Mouse
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unconjugated
Overview
Product Name
P2X7 Recombinant Rabbit Monoclonal Antibody [PSH22-44]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse P2X7 aa 354-595.
Species Reactivity
Mouse
Validated Applications
WB
Target Molecular Weight
Predicted band size: 68 kDa
Positive Control
Mouse brain lysate, Mouse lung tissue lysate.
Conjugation
unconjugated
Clone Number
PSH22-44
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | IHC-Fr | FC | IF-Cell | |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:25,000
Target
Function
P2X purinoceptor 7 is a protein that in humans is encoded by the P2RX7 gene. The product of this gene belongs to the family of purinoceptors for ATP. Multiple alternatively spliced variants which would encode different isoforms have been identified although some fit nonsense-mediated decay criteria. The receptor is found in the central and peripheral nervous systems, in microglia, in macrophages, in uterine endometrium, and in the retina. The P2X7 receptor also serves as a pattern recognition receptor for extracellular ATP-mediated apoptotic cell death, regulation of receptor trafficking, mast cell degranulation, and inflammation. Regarding inflammation, P2X7 receptor induces the NLRP3 inflammasome in myeloid cells and leads to interleukin-1beta release.
Background References
1. Pegoraro A et al. P2X7 Variants in Pathophysiology. Int J Mol Sci. 2024 Jun
2. De Marchi E et al. P2X7 Receptor in Hematological Malignancies. Front Cell Dev Biol. 2021 Mar
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
ATP receptor antibody
P2rx7 antibody
P2X purinoceptor 7 antibody
P2X7 antibody
P2Z receptor antibody
Purinergic receptor antibody
purinergic receptor P2X, ligand gated ion channel, 7 antibody
Images
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☑ Relative expression (RE)
Western blot analysis of P2X7 on different lysates with Rabbit anti-P2X7 antibody (HA724296) at 1/25,000 dilution.
Lane 1: Mouse brain lysate (no heat)
Lane 2: Mouse skeletal muscle lysate (no heat) (negative)
Lane 3: Mouse lung tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 68 kDa
Observed band size: 75 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724296) at 1/25,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Low expression or negative control : Mouse skeletal muscle (PMID: 15254086, PMID: 22415881)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"