FGFR1 Recombinant Rabbit Monoclonal Antibody [PSH22-64]
Catalog# HA724312
FGFR1 Recombinant Rabbit Monoclonal Antibody [PSH22-64]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
FGFR1 Recombinant Rabbit Monoclonal Antibody [PSH22-64]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 92 kDa
Positive Control
A-172 cell lysate, SH-SY5Y cell lysate, U-87 MG cell lysate, mouse kidney tissue, rat kidney tissue.
Conjugation
unconjugated
Clone Number
PSH22-64
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
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| Mouse |
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| Rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000
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IHC-P
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1:1,000
Target
Function
Acidic and basic fibroblast growth factors (FGFs) are members of a family of multifunctional polypeptide growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Like other growth factors, FGFs act by binding and activating specific cell surface receptors. These include the Flg receptor (FGFR-1), the Bek receptor (FGFR-2), FGFR-3, FGFR-4, FGFR-5 and FGFR-6. These receptors usually contain an extracellular ligand-binding region containing three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The gene encoding human Flg maps to chromosome 8p12 and is alternatively spliced to produce several isoforms. Mutations in Flg are associated with Pfeiffer syndrome (a skeletal disorder characterized by craniosynostosis with deviation and enlargement of the thumbs and great toes), brachymesophalangy with phalangeal ankylosis and a varying degree of soft tissue syndactyly. The Flg gene is also involved in chromosomal translocations with ZNF198, CEP110 and FOP, which may lead to stem cell leukemia lymphoma (SCLL).
Background References
1. Lee C et al. VEGF-B prevents excessive angiogenesis by inhibiting FGF2/FGFR1 pathway. Signal Transduct Target Ther. 2023 Aug
2. Auffret L et al. A new subtype of diffuse midline glioma, H3 K27 and BRAF/FGFR1 co-altered: a clinico-radiological and histomolecular characterisation. Acta Neuropathol. 2023 Dec
Subcellular Location
Cell membrane, Nucleus, Cytoplasm, cytosol, Cytoplasmic vesicle.
Synonyms
Basic fibroblast growth factor receptor 1 antibody
bFGF-R-1 antibody
BFGFR antibody
CD331 antibody
CEK antibody
FGFBR antibody
FGFR 1 antibody
FGFR-1 antibody
FGFR1 antibody
FGFR1/PLAG1 fusion antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of FGFR1 on different lysates with Rabbit anti-FGFR1 antibody (HA724312) at 1/5,000 dilution.
Lane 1: A-172 cell lysate
Lane 2: HT-29 cell lysate (negative)
Lane 3: SH-SY5Y cell lysate
Lane 4: U-87 MG cell lysate
Predicted band size: 92 kDa
Observed band size: 92-150 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724312) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-FGFR1 antibody (HA724312) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724312) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-FGFR1 antibody (HA724312) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724312) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
FGFR1 Recombinant Rabbit Monoclonal Antibody [PSH22-64] - BSA and Azide free
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
