TRPA1 Recombinant Rabbit Monoclonal Antibody [PSH23-56]
Catalog# HA724319
TRPA1 Recombinant Rabbit Monoclonal Antibody [PSH23-56]
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WB
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Human
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Green monkey
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unconjugated
Overview
Product Name
TRPA1 Recombinant Rabbit Monoclonal Antibody [PSH23-56]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TRPA1 aa 1-750.
Species Reactivity
Human, Green monkey
Validated Applications
WB
Target Molecular Weight
Predicted band size: 127 kDa
Positive Control
U-2 OS (Human osteosarcoma cells) cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate, SH-SY5Y (Human neuroblastoma cells) cell lysate, IMR-32 (Human neuroblastoma cells) cell lysate, COS-1 (African green monkey kidney fibroblasts) cell lysate.
Conjugation
unconjugated
Clone Number
PSH23-56
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC(Intra) | IF-Cell | |
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| human |
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| mouse |
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| rat |
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| green monkey |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000
Target
Function
Transient receptor potential cation channel, subfamily A, member 1, also known as transient receptor potential ankyrin 1, TRPA1, or The Mustard and Wasabi Receptor, is a protein that in humans is encoded by the TRPA1 (and in mice and rats by the Trpa1) gene. TRPA1 is an ion channel located on the plasma membrane of many human and animal cells. This ion channel is best known as a sensor for pain, cold and itch in humans and other mammals, as well as a sensor for environmental irritants giving rise to other protective responses (tears, airway resistance, and cough).
Background References
1. Tominaga M et al. TRPA1 and thermosensitivity. J Physiol Sci. 2025 Mar
2. Marcotti A et al. TRPA1 modulation by Sigma-1 receptor prevents oxaliplatin-induced painful peripheral neuropathy. Brain. 2023 Feb
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
ANKTM 1 antibody
ANKTM1 antibody
Ankyrin-like with transmembrane domains protein 1 antibody
Transformation sensitive protein p120 antibody
Transformation-sensitive protein p120 antibody
Transient receptor potential cation channel subfamily A member 1 antibody
TRPA 1 antibody
Trpa1 antibody
TRPA1_HUMAN antibody
Images
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Western blot analysis of TRPA1 on different lysates with Rabbit anti-TRPA1 antibody (HA724319) at 1/5,000 dilution.
Lane 1: U-2 OS (Human osteosarcoma cells) cell lysate
Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate
Lane 3: SH-SY5Y (Human neuroblastoma cells) cell lysate
Lane 4: IMR-32 (Human neuroblastoma cells) cell lysate
Lane 5: COS-1 (African green monkey kidney fibroblasts) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 25 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724319, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 127 kDa
Observed band size: 150 kDa -
☑ Knockdown (KD)
Western blot analysis of TRPA1 on different lysates with Rabbit anti-TRPA1 antibody (HA724319) at 1/5,000 dilution.
Lane 1: SH-SY5Y -si WT cell lysate
Lane 2: SH-SY5Y -si TRPA1 cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 20 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724319, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 127 kDa
Observed band size: 150 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"