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CXCR4 Recombinant Rabbit Monoclonal Antibody [PSH22-76]

Usd: 385

Specification

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Catalog# HA724321

CXCR4 Recombinant Rabbit Monoclonal Antibody [PSH22-76]

Application

  • WB

  • IHC-Fr

Reactivity

  • Human

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

CXCR4 Recombinant Rabbit Monoclonal Antibody [PSH22-76]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-Fr

Target Molecular Weight

Predicted band size: 40 kDa

Positive Control

Jurkat (Human T-lymphoblastic cells) cell lysate, HepG2 (Human liver cancer cells) cell lysate, Ramos (Human Burkitt's lymphoma cells) cell lysate, Daudi (Human Burkitt's lymphoma cells) cell lysate.

Conjugation

unconjugated

Clone Number

PSH22-76

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-Fr

  • 1:500

Target

Function

C-X-C chemokine receptor type 4 (CXCR-4) also known as fusin or CD184 (cluster of differentiation 184) is a protein that in humans is encoded by the CXCR4 gene. The protein is a CXC chemokine receptor. CXCR-4 is an alpha-chemokine receptor specific for stromal-derived-factor-1 (SDF-1 also called CXCL12), a molecule endowed with potent chemotactic activity for lymphocytes. CXCR4 is one of several chemokine co-receptors that HIV can use to infect CD4+ T cells. HIV isolates that use CXCR4 are traditionally known as T-cell tropic isolates. Typically, these viruses are found late in infection. It is unclear as to whether the emergence of CXCR4-using HIV is a consequence or a cause of immunodeficiency.

Background References

1. Buck AK et al. CXCR4-targeted theranostics in oncology. Eur J Nucl Med Mol Imaging. 2022 Oct

2. Wu X et al. CXCL12/CXCR4: An amazing challenge and opportunity in the fight against fibrosis. Ageing Res Rev. 2023 Jan

Subcellular Location

Cell membrane, Cell junction, Early endosome, Late endosome, Lysosome.

UNIPROT

SwissProt: P61073 Human

SwissProt: P70658 Mouse

Synonyms

C-X-C chemokine receptor type 4 antibody

CD184 antibody

CD184 antigen antibody

Chemokine (C X C motif) receptor 4 antibody

Chemokine CXC Motif Receptor 4 antibody

CXC-R4 antibody

CXCR-4 antibody

CXCR4 antibody

CXCR4_HUMAN antibody

D2S201E antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CXCR4 on different lysates with Rabbit anti-CXCR4 antibody (<a href="/products/HA724321" style="font-weight: bold;text-decoration: underline;">HA724321</a>) at 1/5,000 dilution.<br /><br />Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate (Unboiled)<br />Lane 2: HepG2 (Human liver cancer cells) cell lysate (negative)<br />Lane 3: Ramos (Human Burkitt's lymphoma cells) cell lysate (Unboiled)<br />Lane 4: Daudi (Human Burkitt's lymphoma cells) cell lysate (Unboiled)<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 6 seconds; ECL: K1801<br /><br />HepG2 is a negative control (PMID: 25363530).<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA724321" style="font-weight: bold;text-decoration: underline;">HA724321</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 40 kDa<br />Observed band size: 30-50 kDa

    ☑ Relative expression (RE)

    Western blot analysis of CXCR4 on different lysates with Rabbit anti-CXCR4 antibody (HA724321) at 1/5,000 dilution.

    Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate (Unboiled)
    Lane 2: HepG2 (Human liver cancer cells) cell lysate (negative)
    Lane 3: Ramos (Human Burkitt's lymphoma cells) cell lysate (Unboiled)
    Lane 4: Daudi (Human Burkitt's lymphoma cells) cell lysate (Unboiled)

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 6 seconds; ECL: K1801

    HepG2 is a negative control (PMID: 25363530).

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724321, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 40 kDa
    Observed band size: 30-50 kDa

  • Application: Immunofluorescence (IHC-Fr)<br /><br />Species: Mouse<br />Tissue: E14.0 embryonic brain<br />Sample: Frozen section<br /><br />Antigen retrieval: Not required<br /><br />Wash buffer: 1× PBS<br />Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA724321" style="font-weight: bold;text-decoration: underline;">HA724321</a>, 1/500, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: E14.0 embryonic brain
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× PBS
    Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
    Primary antibody: HA724321, 1/500, overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"