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Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [PSH23-93]

Usd: 385

Specification

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Catalog# HA724408

Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [PSH23-93]

Application

  • WB

  • IHC-P

  • IF-Tissue

  • IP

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [PSH23-93]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Mannose Receptor(CD206) aa 1-1,389.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IF-Tissue, IP

Target Molecular Weight

Predicted band size: 166 kDa

Positive Control

THP-1 treated with 20ng/ml PMA for 24 hours then treated with 20ng/ml IL-4 for 48 hours cell lysate.

Conjugation

unconjugated

Clone Number

PSH23-93

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:1,000-1:3,000

  • IF-Tissue

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

CD206, also known as macrophage mannose receptor type C (MMR or MRC1), is a type I membrane receptor protein. It is an phagocytic and endocytic receptor that can recognize carbohydrate ligands in target molecules. The extracellular portion of the protein includes eight C-type carbohydrate recognition domains (CRD) which are clustered together to achieve higher affinity binding to saccharides. CD206 is found on macrophages and on endothelial cells of the liver and is the only known example of a C-type lectin that contains multiple C-type CRDs. CD206 mediates the endocytosis of glycoproteins by macrophages and binds high-mannose structures on the surface of potentially pathogenic viruses, fungi and bacteria enabling them to be neutralized by phagocytic engulfment. During inflammation, CD206 is crucial for rapid clearance of several mannose-bearing serum glycoproteins but does not regulate the initiation of inflammation. CD206 is primarily expressed in mature tissue macrophages and immature dendritic cells, as well as hepatic and lymphatic endothelial cells, retinal pigmental epithelium (RPE) and mesangial cells.

Background References

1. Ray A et al. Targeting CD206+ macrophages disrupts the establishment of a key antitumor immune axis. J Exp Med. 2025 Jan

2. Pommerolle L et al. CD206(+) macrophages are relevant non-invasive imaging biomarkers and therapeutic targets in experimental lung fibrosis. Thorax. 2024 Nov

Subcellular Location

Endosome membrane, Cell membrane.

UNIPROT

SwissProt: P22897 Human

Synonyms

bA541I19.1 antibody

C type lectin domain family 13 member D antibody

C-type lectin domain family 13 member D antibody

CD 206 antibody

CD206 antibody

CD206 antigen antibody

CLEC13D antibody

CLEC13DL antibody

Macrophage mannose receptor 1 antibody

Macrophage mannose receptor 1 like protein 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Mannose Receptor(CD206) on different lysates with Rabbit anti-Mannose Receptor(CD206) antibody (<a href="/products/HA724408" style="font-weight: bold;text-decoration: underline;">HA724408</a>) at 1/5,000 dilution.<br /><br />Lane 1: THP-1 (Human acute monoblastic leukemia cells) cell lysate<br />Lane 2: THP-1 treated with 20ng/ml PMA for 24 hours then treated with 20ng/ml IL-4 for 48 hours cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 20 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA724408" style="font-weight: bold;text-decoration: underline;">HA724408</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 166 kDa<br />Observed band size: 250 kDa

    ☑ Cell treatment (CT)

    Western blot analysis of Mannose Receptor(CD206) on different lysates with Rabbit anti-Mannose Receptor(CD206) antibody (HA724408) at 1/5,000 dilution.

    Lane 1: THP-1 (Human acute monoblastic leukemia cells) cell lysate
    Lane 2: THP-1 treated with 20ng/ml PMA for 24 hours then treated with 20ng/ml IL-4 for 48 hours cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724408, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 166 kDa
    Observed band size: 250 kDa

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Colon <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724408" style="font-weight: bold;text-decoration: underline;">HA724408</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Colon
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724408, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Liver <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724408" style="font-weight: bold;text-decoration: underline;">HA724408</a>, 1/3,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724408, 1/3,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Lung <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724408" style="font-weight: bold;text-decoration: underline;">HA724408</a>, 1/3,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Lung
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724408, 1/3,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunofluorescence (IF-tissue)<br /><br />Species: Human<br />Tissue: Liver<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.<br />Primary antibody: <a href="/products/HA724408" style="font-weight: bold;text-decoration: underline;">HA724408</a>, 1/1,000, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Human
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
    Primary antibody: HA724408, 1/1,000, overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Immunoprecipitation (IP)<br /><br />Mannose Receptor(CD206) was immunoprecipitated in 0.2 mg THP-1 (Human acute monoblastic leukemia cell) treated with 20ng/mL TPA for 24 hours then treated with 20ng/mL IL-4 for 48 hours cell lysate with <a href="/products/HA724408" style="font-weight: bold;text-decoration: underline;">HA724408</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA724408" style="font-weight: bold;text-decoration: underline;">HA724408</a> at 1/5,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs&reg; VHH(HRP) (<a href="/products/025-100-005" style="font-weight: bold;text-decoration: underline;">025-100-005</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: THP-1 treated with 20ng/mL TPA for 24 hours then treated with 20ng/mL IL-4 for 48 hours cell lysate<br />Lane 2: <a href="/products/HA724408" style="font-weight: bold;text-decoration: underline;">HA724408</a> IP in THP-1 treated with 20ng/mL TPA for 24 hours then treated with 20ng/mL IL-4 for 48 hours cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA724408" style="font-weight: bold;text-decoration: underline;">HA724408</a> in THP-1 treated with 20ng/mL TPA for 24 hours then treated with 20ng/mL IL-4 for 48 hours cell lysate<br /><br />Exposure time: 3 minutes<br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br /><br />Primary dilution: <a href="/products/HA724408" style="font-weight: bold;text-decoration: underline;">HA724408</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), 2 hours at room temperature<br /><br />Predicted band size: 166 kDa<br />Observed band size: 250 kDa

    ☑ Cell treatment (CT)

    Immunoprecipitation (IP)

    Mannose Receptor(CD206) was immunoprecipitated in 0.2 mg THP-1 (Human acute monoblastic leukemia cell) treated with 20ng/mL TPA for 24 hours then treated with 20ng/mL IL-4 for 48 hours cell lysate with HA724408 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724408 at 1/5,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: THP-1 treated with 20ng/mL TPA for 24 hours then treated with 20ng/mL IL-4 for 48 hours cell lysate
    Lane 2: HA724408 IP in THP-1 treated with 20ng/mL TPA for 24 hours then treated with 20ng/mL IL-4 for 48 hours cell lysate
    Lane 3: Rabbit IgG instead of HA724408 in THP-1 treated with 20ng/mL TPA for 24 hours then treated with 20ng/mL IL-4 for 48 hours cell lysate

    Exposure time: 3 minutes
    Blocking: 5% NFDM/TBST, 1 hour at room temperature

    Primary dilution: HA724408, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature

    Predicted band size: 166 kDa
    Observed band size: 250 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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