Human/Monkey Apolipoprotein CIII Recombinant Rabbit Monoclonal Antibody [PSH24-27] - BSA and Azide free (Detector)

Sandwich ELISA analysis of Human Apolipoprotein CIII matched pair antibodies

Capture: HA724433, Human/Monkey Apolipoprotein CIII Rabbit mAb [PSH24-26]
Detector: HA724434, Human/Monkey Apolipoprotein CIII Rabbit mAb [PSH24-27]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724433) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Apolipoprotein CIII protein (HA211274) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA724434, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Apolipoprotein CIII in HepG2 cell culture supernatant.

Capture: HA724433, Human/Monkey Apolipoprotein CIII Rabbit mAb [PSH24-26]
Detector: HA724434, Human/Monkey Apolipoprotein CIII Rabbit mAb [PSH24-27]

The concentrations of Apolipoprotein CIII were measured in duplicates, interpolated from the Apolipoprotein CIII standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein CIII concentration was determined to be 2,809 pg/ml in HepG2 cell culture supernatant.

Interpolated concentrations of native Apolipoprotein CIII in human serum samples.

Capture: HA724433, Human/Monkey Apolipoprotein CIII Rabbit mAb [PSH24-26]
Detector: HA724434, Human/Monkey Apolipoprotein CIII Rabbit mAb [PSH24-27]

The concentrations of Apolipoprotein CIII were measured in duplicates, interpolated from the Apolipoprotein CIII standard curve and corrected for sample dilution. Undiluted samples are human serum 0.005%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein CIII concentration was determined to be 71.3 μg/ml in human serum.

Interpolated concentrations of native Apolipoprotein CIII in Rhesus monkey serum samples.

Capture: HA724433, Human/Monkey Apolipoprotein CIII Rabbit mAb [PSH24-26]
Detector: HA724434, Human/Monkey Apolipoprotein CIII Rabbit mAb [PSH24-27]

The concentrations of Apolipoprotein CIII were measured in duplicates, interpolated from the Apolipoprotein CIII standard curve and corrected for sample dilution. Undiluted samples are Rhesus monkey serum 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein CIII concentration was determined to be 191.5 ng/ml in Rhesus monkey serum.

Interpolated concentrations of spiked Apolipoprotein CIII in human cell culture media samples.

Capture: HA724433, Human/Monkey Apolipoprotein CIII Rabbit mAb [PSH24-26]
Detector: HA724434, Human/Monkey Apolipoprotein CIII Rabbit mAb [PSH24-27]

The concentrations of Apolipoprotein CIII were measured in duplicates, interpolated from the Apolipoprotein CIII standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).