Human/Monkey IGFBP2 Recombinant Rabbit Monoclonal Antibody [PSH25-34] - BSA and Azide free (Detector)
Catalog# HA724525
Human/Monkey IGFBP2 Recombinant Rabbit Monoclonal Antibody [PSH25-34] - BSA and Azide free (Detector)
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ELISA(Det)
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Human
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Cynomolgus monkey
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312.5-40,000 pg/mL
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6.76
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unconjugated
Overview
Product Name
Human/Monkey IGFBP2 Recombinant Rabbit Monoclonal Antibody [PSH25-34] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human IGFBP2 aa 36-325 (HA211103).
Species Reactivity
Human, Cynomolgus monkey
Validated Applications
ELISA(Det)
Positive Control
Recombinant Human IGFBP2 protein (HA211103).
Conjugation
unconjugated
Clone Number
PSH25-34
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH25-33] to Human/Monkey IGFBP2 (Capture) (HA724524) and Recombinant Human IGFBP2 protein (HA211103) as the standard. The reference range value is 312.5-40,000 pg/mL.
Target
Function
The Insulin-like growth factor-binding proteins (IGFBPs), a family of homologous proteins that have co-evolved with the IGFs, serve not only as shuttle molecules for the soluble IGFs, but also confer a level of regulation to the IGF signaling system. Physical association of the IGFBPs with IGF influences the bio-availability of the growth factors, and their concentration and distribution in the extracellular environment. The IGFBPs also appear to have biological activity independent of the IGFs. Seven IGFBPs have been described, each differing in their tissue distribution, half-lives and modulation of IGF interactions with their receptors. IGFBP1 is negatively regulated by Insulin production. The IGFBP1 gene is expressed at a high level during fetal liver development and in response to nutritional changes and diabetes. IGFBP2, which may function as a chaperone, escorting IGFs to their target tissues, is expressed in several human tissues including fetal eye and fetal brain.
Background References
1. Chin C et al. Loss of IGFBP2 mediates alveolar type 2 cell senescence and promotes lung fibrosis. Cell Rep Med. 2023 Mar
2. Lin YH et al. IGFBP2 expressing midlobular hepatocytes preferentially contribute to liver homeostasis and regeneration. Cell Stem Cell. 2023 May
Subcellular Location
Secreted.
UNIPROT
Synonyms
BP 2 antibody
BP2 antibody
IBP 2 antibody
IBP-2 antibody
IBP2 antibody
IBP2_HUMAN antibody
IGF binding protein 2 antibody
IGF BP53 antibody
IGF-binding protein 2 antibody
IGFBP 2 antibody
ExpandImages
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Sandwich ELISA analysis of Human IGFBP2 matched pair antibodies
Capture: HA724524, Human/Monkey IGFBP2 Rabbit mAb [PSH25-33]
Detector: HA724525, Human/Monkey IGFBP2 Rabbit mAb [PSH25-34]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724524) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IGFBP2 protein (HA211103) starting from 40,000 pg/mL to 0 pg/mL and detect antibody (HA724525, Biotin, 0.05 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentrations of native IGFBP2 in human and monkey samples.
Capture: HA724524, Human/Monkey IGFBP2 Rabbit mAb [PSH25-33]
Detector: HA724525, Human/Monkey IGFBP2 Rabbit mAb [PSH25-34]
The concentrations of IGFBP2 were measured in duplicates, interpolated from the IGFBP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 554,351 pg/mL in Cynomolgus serum, 213,887 pg/mL in human serum and 55,588 pg/mL in T-47D cell supernatant. -
Interpolated concentrations of spiked IGFBP2 in human cell culture media samples.
Capture: HA724524, Human/Monkey IGFBP2 Rabbit mAb [PSH25-33]
Detector: HA724525, Human/Monkey IGFBP2 Rabbit mAb [PSH25-34]
The concentrations of IGFBP2 were measured in duplicates, interpolated from the IGFBP2 standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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