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Osteoprotegerin Recombinant Rabbit Monoclonal Antibody [PSH26-52]

Usd: 385

Specification

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Catalog# HA724629

Osteoprotegerin Recombinant Rabbit Monoclonal Antibody [PSH26-52]

Application

  • WB

  • IHC-P

  • FC(Intra)

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Osteoprotegerin Recombinant Rabbit Monoclonal Antibody [PSH26-52]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse Osteoprotegerin aa 1-401.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC(Intra)

Target Molecular Weight

Predicted band size: 46 kDa

Positive Control

K-562 (Human chronic myelogenous leukemia cell) cell lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Rat liver tissue lysate, Rat kidney tissue lysate.

Conjugation

unconjugated

Clone Number

PSH26-52

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:1,000

  • FC(Intra)

  • 1:1,000

Target

Function

Osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor (OCIF) or tumour necrosis factor receptor superfamily member 11B (TNFRSF11B), is a cytokine receptor of the tumour necrosis factor (TNF) receptor superfamily encoded by the TNFRSF11B gene. OPG was first discovered as a novel secreted TNFR related protein that played a role in the regulation of bone density and later for its role as a decoy receptor for receptor activator of nuclear factor kappa-B ligand (RANKL). OPG also binds to TNF-related apoptosis-inducing ligand (TRAIL) and inhibits TRAIL induced apoptosis of specific cells, including tumour cells. Other OPG ligands include syndecan-1, glycosaminoglycans, von Willebrand factor, and factor VIII-von Willebrand factor complex. OPG has been identified as having a role in tumour growth and metastasis, heart disease, immune system development and signalling, mental health, diabetes, and the prevention of pre-eclampsia and osteoporosis during pregnancy.

Background References

1. Dutka M et al. Osteoprotegerin and RANKL-RANK-OPG-TRAIL signalling axis in heart failure and other cardiovascular diseases. Heart Fail Rev. 2022 Jul

2. Xu M et al. Association between Osteoprotegerin rs2073618 polymorphism and peri-implantitis susceptibility: a meta-analysis. BMC Oral Health. 2022 Dec

Subcellular Location

Secreted.

UNIPROT

SwissProt: O00300 Human

SwissProt: O08712 Mouse

SwissProt: O08727 Rat

Synonyms

MGC29565 antibody

OCIF antibody

OPG antibody

Osteoclastogenesis inhibitory factor antibody

Osteoprotegerin antibody

PDB5 antibody

TNF receptor superfamily member 11b antibody

TNFRSF 11B antibody

TNFRSF11B antibody

TR 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Osteoprotegerin on different lysates with Rabbit anti-Osteoprotegerin antibody (<a href="/products/HA724629" style="font-weight: bold;text-decoration: underline;">HA724629</a>) at 1/5,000 dilution. <br /> <br />Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate <br />Lane 2: Mouse liver tissue lysate <br />Lane 3: Mouse kidney tissue lysate <br />Lane 4: Mouse skeletal muscle tissue lysate <br />Lane 5: Rat liver tissue lysate <br />Lane 6: Rat kidney tissue lysate <br />Lane 7: Rat skeletal muscle tissue lysate <br /> <br />Lysates/proteins at 20 µg/Lane. <br />Exposure time: 3 minutes; ECL: K1801 <br /> <br />Negative expression of Osteoprotegerin/TNFRSF11B protein in skeletal muscle is consistent with the predicted expression pattern. <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724629" style="font-weight: bold;text-decoration: underline;">HA724629</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 46 kDa <br />Observed band size: 56 kDa

    ☑ Relative expression (RE)

    Western blot analysis of Osteoprotegerin on different lysates with Rabbit anti-Osteoprotegerin antibody (HA724629) at 1/5,000 dilution.

    Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate
    Lane 2: Mouse liver tissue lysate
    Lane 3: Mouse kidney tissue lysate
    Lane 4: Mouse skeletal muscle tissue lysate
    Lane 5: Rat liver tissue lysate
    Lane 6: Rat kidney tissue lysate
    Lane 7: Rat skeletal muscle tissue lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 3 minutes; ECL: K1801

    Negative expression of Osteoprotegerin/TNFRSF11B protein in skeletal muscle is consistent with the predicted expression pattern.

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724629, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 46 kDa
    Observed band size: 56 kDa

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Kidney <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724629" style="font-weight: bold;text-decoration: underline;">HA724629</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Kidney
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724629, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Liver <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724629" style="font-weight: bold;text-decoration: underline;">HA724629</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724629, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Kidney <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724629" style="font-weight: bold;text-decoration: underline;">HA724629</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Kidney
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724629, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Skeletal muscle <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724629" style="font-weight: bold;text-decoration: underline;">HA724629</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature. <br /> <br />Negative expression of Osteoprotegerin protein in skeletal muscle is consistent with the predicted expression pattern.

    ☑ Relative expression (RE)

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Skeletal muscle
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724629, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

    Negative expression of Osteoprotegerin protein in skeletal muscle is consistent with the predicted expression pattern.

  • Application: Flow Cytometry (Intra)<br /><br />Species: Human<br />Sample: K-562 (Human chronic myelogenous leukemia cell)<br /><br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.<br />Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.<br />Antibody dilution buffer: 1x PBS.<br /><br />Primary antibody: <a href="/products/HA724629" style="font-weight: bold;text-decoration: underline;">HA724629</a> (1/1,000, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature.

    Application: Flow Cytometry (Intra)

    Species: Human
    Sample: K-562 (Human chronic myelogenous leukemia cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA724629 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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