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CDCP1 Recombinant Rabbit Monoclonal Antibody [PSH26-74]

Usd: 385

Specification

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Catalog# HA724639

CDCP1 Recombinant Rabbit Monoclonal Antibody [PSH26-74]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

CDCP1 Recombinant Rabbit Monoclonal Antibody [PSH26-74]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human CDCP1 aa 1-667.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC

Target Molecular Weight

Predicted band size: 93 kDa

Positive Control

HCT 116 (Human colon cancer cell) cell lysate, HT-29 (Human colorectal adenocarcinoma cell) cell lysate, COLO205 (Human colon adenocarcinoma cell) cell lysate, MDA-MB-231 (Human breast cancer cell) cell lysate, Mouse colon tissue lysate, Mouse small intestine tissue lysate, Rat colon tissue lysate, Rat small intestine tissue lysate.

Conjugation

unconjugated

Clone Number

PSH26-74

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P FC IF-Cell
human
Tested Tested
Tested Tested
Tested Tested
Not Recommended
mouse
Tested Tested
 
Not Recommended
Not Recommended
rat
Tested Tested
Tested Tested
 
Not Recommended
green monkey
Not Recommended
 
 
Not Recommended

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:1,000-1:2,000

  • FC

  • 1:1,000

Target

Function

CDCP1 (CUB-domain-containing protein 1) contains three extracellular CUB domains, a transmembrane domain, and two putative cytoplasmic tyrosine phosphorylation sites. Phosphorylation of the gp140 and p80 proteins is mediated by Src family kinases at various tyrosine residues, including Tyr 734. PTP family members mediate the desphosphorylation of CDCP1. The conversion of gp140 to p80 prolongs the phosphorylation state, which may affect signaling in epithelial wounds. CDCP1 acts as a marker for hematopoetic cells and also exhibits high expression in metastatic colon and breast tumors.

Background References

1. Khan T et al. CUB Domain-Containing Protein 1 (CDCP1) is a rational target for the development of imaging tracers and antibody-drug conjugates for cancer detection and therapy. Theranostics. 2022 Oct

2. Huang H et al. Targeting CDCP1 boost CD8+ T cells-mediated cytotoxicity in cervical cancer via the JAK/STAT signaling pathway. J Immunother Cancer. 2024 Oct

Subcellular Location

Cell membrane. Secreted.

UNIPROT

SwissProt: Q9H5V8 Human

SwissProt: Q5U462 Mouse

Entrez Gene: 301082 Rat

Synonyms

9030022E12Rik antibody

AA409659 antibody

CD 318 antibody

CD318 antibody

CD318 antigen antibody

CDCP 1 antibody

Cdcp1 antibody

CDCP1_HUMAN antibody

CUB domain containing protein 1 antibody

CUB domain-containing protein 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CDCP1 on different lysates with Rabbit anti-CDCP1 antibody (<a href="/products/HA724639" style="font-weight: bold;text-decoration: underline;">HA724639</a>) at 1/5,000 dilution. <br /> <br />Lane 1: HCT 116 (Human colon cancer cell) cell lysate (15 µg/Lane) <br />Lane 2: HT-29 (Human colorectal adenocarcinoma cell) cell lysate (15 µg/Lane) <br />Lane 3: COLO205 (Human colon adenocarcinoma cell) cell lysate (15 µg/Lane) <br />Lane 4: MDA-MB-231 (Human breast cancer cell) cell lysate (15 µg/Lane) <br />Lane 5: MCF7 (Human breast cancer cell) cell lysate (15 µg/Lane) <br />Lane 6: Mouse colon tissue lysate (20 µg/Lane) <br />Lane 7: Mouse small intestine tissue lysate (20 µg/Lane) <br />Lane 8: Rat colon tissue lysate (20 µg/Lane) <br />Lane 9: Rat small intestine tissue lysate (20 µg/Lane) <br /> <br />Exposure time: 25 seconds; ECL: K1801 <br /> <br />Negative control: MCF7 (PMID: 20551327, 24055141). <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724639" style="font-weight: bold;text-decoration: underline;">HA724639</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 92.9 kDa <br />Observed band size: 140, 70 kDa <br /> <br />The molecular weight is consistent with the reference (PMID: 20551327).

    ☑ Relative expression (RE)

    Western blot analysis of CDCP1 on different lysates with Rabbit anti-CDCP1 antibody (HA724639) at 1/5,000 dilution.

    Lane 1: HCT 116 (Human colon cancer cell) cell lysate (15 µg/Lane)
    Lane 2: HT-29 (Human colorectal adenocarcinoma cell) cell lysate (15 µg/Lane)
    Lane 3: COLO205 (Human colon adenocarcinoma cell) cell lysate (15 µg/Lane)
    Lane 4: MDA-MB-231 (Human breast cancer cell) cell lysate (15 µg/Lane)
    Lane 5: MCF7 (Human breast cancer cell) cell lysate (15 µg/Lane)
    Lane 6: Mouse colon tissue lysate (20 µg/Lane)
    Lane 7: Mouse small intestine tissue lysate (20 µg/Lane)
    Lane 8: Rat colon tissue lysate (20 µg/Lane)
    Lane 9: Rat small intestine tissue lysate (20 µg/Lane)

    Exposure time: 25 seconds; ECL: K1801

    Negative control: MCF7 (PMID: 20551327, 24055141).

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724639, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 92.9 kDa
    Observed band size: 140, 70 kDa

    The molecular weight is consistent with the reference (PMID: 20551327).

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Breast cancer <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724639" style="font-weight: bold;text-decoration: underline;">HA724639</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Breast cancer
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724639, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Colon cancer <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724639" style="font-weight: bold;text-decoration: underline;">HA724639</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Colon cancer
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724639, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Human<br />Tissue: Colon<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA724639" style="font-weight: bold;text-decoration: underline;">HA724639</a>, 1/2,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Colon
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724639, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Application: Flow Cytometry <br /> <br />Species: Human <br />Sample: MCF7 (Human breast cancer cells) (Left, Negative control)/HCT 116 (Human colon cancer cells) (Right, Positive control) <br /> <br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. <br />Antibody dilution buffer: 1x PBS. <br /> <br />Primary antibody: <a href="/products/HA724639" style="font-weight: bold;text-decoration: underline;">HA724639</a> (1/1,000, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature. <br /> <br />Negative control: MCF7 ((PMID: 20551327, 24055141).

    ☑ Relative expression (RE)

    Application: Flow Cytometry

    Species: Human
    Sample: MCF7 (Human breast cancer cells) (Left, Negative control)/HCT 116 (Human colon cancer cells) (Right, Positive control)

    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA724639 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

    Negative control: MCF7 ((PMID: 20551327, 24055141).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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