CDCP1 Recombinant Rabbit Monoclonal Antibody [PSH26-74]
Catalog# HA724639
CDCP1 Recombinant Rabbit Monoclonal Antibody [PSH26-74]
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WB
-
IHC-P
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FC
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Human
-
Mouse
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Rat
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unconjugated
Overview
Product Name
CDCP1 Recombinant Rabbit Monoclonal Antibody [PSH26-74]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CDCP1 aa 1-667.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
Predicted band size: 93 kDa
Positive Control
HCT 116 (Human colon cancer cell) cell lysate, HT-29 (Human colorectal adenocarcinoma cell) cell lysate, COLO205 (Human colon adenocarcinoma cell) cell lysate, MDA-MB-231 (Human breast cancer cell) cell lysate, Mouse colon tissue lysate, Mouse small intestine tissue lysate, Rat colon tissue lysate, Rat small intestine tissue lysate.
Conjugation
unconjugated
Clone Number
PSH26-74
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
IHC-P
-
1:1,000
-
FC
-
1:1,000
Target
Function
CDCP1 (CUB-domain-containing protein 1) contains three extracellular CUB domains, a transmembrane domain, and two putative cytoplasmic tyrosine phosphorylation sites. Phosphorylation of the gp140 and p80 proteins is mediated by Src family kinases at various tyrosine residues, including Tyr 734. PTP family members mediate the desphosphorylation of CDCP1. The conversion of gp140 to p80 prolongs the phosphorylation state, which may affect signaling in epithelial wounds. CDCP1 acts as a marker for hematopoetic cells and also exhibits high expression in metastatic colon and breast tumors.
Background References
1. Khan T et al. CUB Domain-Containing Protein 1 (CDCP1) is a rational target for the development of imaging tracers and antibody-drug conjugates for cancer detection and therapy. Theranostics. 2022 Oct
2. Huang H et al. Targeting CDCP1 boost CD8+ T cells-mediated cytotoxicity in cervical cancer via the JAK/STAT signaling pathway. J Immunother Cancer. 2024 Oct
Subcellular Location
Cell membrane. Secreted.
Synonyms
9030022E12Rik antibody
AA409659 antibody
CD 318 antibody
CD318 antibody
CD318 antigen antibody
CDCP 1 antibody
Cdcp1 antibody
CDCP1_HUMAN antibody
CUB domain containing protein 1 antibody
CUB domain-containing protein 1 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of CDCP1 on different lysates with Rabbit anti-CDCP1 antibody (HA724639) at 1/5,000 dilution.
Lane 1: HCT 116 (Human colon cancer cell) cell lysate (15 µg/Lane)
Lane 2: HT-29 (Human colorectal adenocarcinoma cell) cell lysate (15 µg/Lane)
Lane 3: COLO205 (Human colon adenocarcinoma cell) cell lysate (15 µg/Lane)
Lane 4: MDA-MB-231 (Human breast cancer cell) cell lysate (15 µg/Lane)
Lane 5: MCF7 (Human breast cancer cell) cell lysate (15 µg/Lane)
Lane 6: Mouse colon tissue lysate (20 µg/Lane)
Lane 7: Mouse small intestine tissue lysate (20 µg/Lane)
Lane 8: Rat colon tissue lysate (20 µg/Lane)
Lane 9: Rat small intestine tissue lysate (20 µg/Lane)
Exposure time: 25 seconds; ECL: K1801
Negative control: MCF7 (PMID: 20551327, 24055141).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724639, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 92.9 kDa
Observed band size: 140, 70 kDa
The molecular weight is consistent with the reference (PMID: 20551327). -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Breast cancer
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724639, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Colon cancer
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724639, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Colon
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724639, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
Application: Flow Cytometry
Species: Human
Sample: MCF7 (Human breast cancer cells) (Left, Negative control)/HCT 116 (Human colon cancer cells) (Right, Positive control)
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724639 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Negative control: MCF7 ((PMID: 20551327, 24055141).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CDCP1 Mouse Monoclonal Antibody [A10F10]
Application: WB
Reactivity: Human,Mouse
Conjugate: unconjugated
