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Phospho-IRF3 (S396) Recombinant Rabbit Monoclonal Antibody [PSH27-05]

Usd: 385

Specification

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Catalog# HA724666

Phospho-IRF3 (S396) Recombinant Rabbit Monoclonal Antibody [PSH27-05]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Phospho-IRF3 (S396) Recombinant Rabbit Monoclonal Antibody [PSH27-05]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser388 of moues IRF3.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 47 kDa

Positive Control

C2C12 transfected with 2.5μg/mL Poly(I:C) for 6 hours cell lysate, NIH/3T3 transfected with 2.5μg/mL Poly(I:C) for 6 hours cell lysate, J774A.1 transfected with 2.5μg/mL Poly(I:C) for 6 hours cell lysate.

Conjugation

unconjugated

Clone Number

PSH27-05

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:2,000-1:5,000

Target

Function

Interferon regulatory factor 3, also known as IRF3, is an interferon regulatory factor. IRF3 is a member of the interferon regulatory transcription factor (IRF) family. IRF3 was originally discovered as a homolog of IRF1 and IRF2. IRF3 has been further characterized and shown to contain several functional domains including a nuclear export signal, a DNA-binding domain, a C-terminal IRF association domain and several regulatory phosphorylation sites. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. The complex translocates into the nucleus for the transcriptional activation of interferons alpha and beta, and further interferon-induced genes.

Background References

1. Al Hamrashdi M et al. Regulation of IRF3 activation in human antiviral signaling pathways. Biochem Pharmacol. 2022 Jun

2. Yan S et al. IRF3 reduces adipose thermogenesis via ISG15-mediated reprogramming of glycolysis. J Clin Invest. 2021 Apr

Subcellular Location

Cytoplasm, Nucleus, Mitochondrion.

UNIPROT

SwissProt: Q14653 Human

SwissProt: P70671 Mouse

Entrez Gene: 292892 Rat

Synonyms

IIAE7 antibody

Interferon regulatory factor 3 antibody

IRF 3 antibody

IRF-3 antibody

IRF3 antibody

IRF3_HUMAN antibody

MGC94729 antibody

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-IRF3 (S396) on different lysates with Rabbit anti-Phospho-IRF3 (S396) antibody (<a href="/products/HA724666" style="font-weight: bold;text-decoration: underline;">HA724666</a>) at 1/5,000 dilution.<br /><br />Lane 1: C2C12 (Mouse myoblast) cell lysate<br />Lane 2: C2C12 transfected with 2.5μg/mL Poly(I:C) for 6 hours cell lysate<br />Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate<br />Lane 4: NIH/3T3 transfected with 2.5μg/mL Poly(I:C) for 6 hours cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 56 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA724666" style="font-weight: bold;text-decoration: underline;">HA724666</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 47 kDa<br />Observed band size: 47 kDa

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-IRF3 (S396) on different lysates with Rabbit anti-Phospho-IRF3 (S396) antibody (HA724666) at 1/5,000 dilution.

    Lane 1: C2C12 (Mouse myoblast) cell lysate
    Lane 2: C2C12 transfected with 2.5μg/mL Poly(I:C) for 6 hours cell lysate
    Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate
    Lane 4: NIH/3T3 transfected with 2.5μg/mL Poly(I:C) for 6 hours cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 56 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724666, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-IRF3 (S396) on different lysates with Rabbit anti-Phospho-IRF3 (S396) antibody (<a href="/products/HA724666" style="font-weight: bold;text-decoration: underline;">HA724666</a>) at 1/5,000 dilution.<br /><br />Lane 1: J774A.1 (Mouse monocyte macrophage) cell lysate<br />Lane 2: J774A.1 transfected with 2.5μg/mL Poly(I:C) for 6 hours cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 1 minute 2 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA724666" style="font-weight: bold;text-decoration: underline;">HA724666</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 47 kDa<br />Observed band size: 47 kDa

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-IRF3 (S396) on different lysates with Rabbit anti-Phospho-IRF3 (S396) antibody (HA724666) at 1/5,000 dilution.

    Lane 1: J774A.1 (Mouse monocyte macrophage) cell lysate
    Lane 2: J774A.1 transfected with 2.5μg/mL Poly(I:C) for 6 hours cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 1 minute 2 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724666, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Testis <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724666" style="font-weight: bold;text-decoration: underline;">HA724666</a>, 1/2,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    ☑ Cell treatment (CT)

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Testis
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724666, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Testis <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724666" style="font-weight: bold;text-decoration: underline;">HA724666</a>, 1/5,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    ☑ Cell treatment (CT)

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Testis
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724666, 1/5,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Testis <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724666" style="font-weight: bold;text-decoration: underline;">HA724666</a>, 1/5,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    ☑ Cell treatment (CT)

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Testis
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724666, 1/5,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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