Desmoglein 3 Recombinant Rabbit Monoclonal Antibody [PSH27-06]
Catalog# HA724667
Desmoglein 3 Recombinant Rabbit Monoclonal Antibody [PSH27-06]
-
WB
-
IF-Cell
-
IHC-P
-
FC
-
Human
-
unconjugated
Overview
Product Name
Desmoglein 3 Recombinant Rabbit Monoclonal Antibody [PSH27-06]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Desmoglein 3 aa 301-615.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 108 kDa
Positive Control
A431 (Human epidermoid carcinoma skin squamous cell) cell lysate, HaCaT (Human immortalized keratinocyte) cell lysate.
Conjugation
unconjugated
Clone Number
PSH27-06
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | |
|---|---|---|---|---|
| human |
|
|
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
IF-Cell
-
1:500
-
IHC-P
-
1:200-1:1,000
-
FC
-
1:2,000
Target
Function
Desmoglein-3 is a protein that in humans is encoded by the DSG3 gene. In the skin epidermis Desmoglein-3 is expressed in the basal lower layers of the epidermis, and dominates in terms of expression on mucosal surfaces compared to Desmoglein-1. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, four desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein, along with Desmoglein-1, has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris. The mucosal dominant form of pemphigus vulgaris only involves antibodies against Desmoglein-3 and causes mucosal erosions, but no skin lesions. Desmoglein-3 serves as a prognostic marker of Esophageal Squamous Cell Carcinoma (ESCC), and may even be involved in the progression of ESCC.
Background References
1. Dworschak J et al. Mapping of B cell epitopes on desmoglein 3 in pemphigus vulgaris patients by the use of overlapping peptides.J Dermatol Sci 65(2):102-9 (2012).
2. Savci-Heijink CD et al. The role of desmoglein-3 in the diagnosis of squamous cell carcinoma of the lung.Am J Pathol. 174(5):1629-37 (2009).
Subcellular Location
Cell membrane, Cell junction, desmosome, Cytoplasm, tight junction.
UNIPROT
Synonyms
130 kD pemphigus vulgaris antigen antibody
130 kDa pemphigus vulgaris antigen antibody
Balding antibody
Cadherin family member 6 antibody
CDHF 6 antibody
CDHF6 antibody
Desmoglein 3 antibody
Desmoglein 3 precursor antibody
Desmoglein-3 antibody
Desmoglein3 antibody
ExpandImages
-
☑ Relative expression (RE)
Western blot analysis of Desmoglein 3 on different lysates with Rabbit anti-Desmoglein 3 antibody (HA724667) at 1/5,000 dilution.
Lane 1: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate
Lane 2: A549 (Human lung adenocarcinoma cell) cell lysate
Lane 3: HaCaT (Human immortalized keratinocyte) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 20 seconds; ECL: K1801
Low expression of Desmoglein 3 protein in A549 cells is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724667, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 108 kDa
Observed band size: 150 kDa -
☑ Relative expression (RE)
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: HaCaT (Human immortalized keratinocyte) / A549 (Human lung adenocarcinoma cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724667, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
Low expression of Desmoglein 3 protein in A549 cells is consistent with the predicted expression pattern. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Skin
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724667, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Esophagus
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724667, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Lung squamous cell carcinoma
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724667, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
Application: Flow Cytometry
Species: Human
Sample: A549 (Human lung adenocarcinoma cell) (Left) / HaCaT (Human immortalized keratinocyte) (Right)
Blocking: 1% BSA + 10% normal goat serum , 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724667 (1/2,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Low expression of Desmoglein 3 protein in A549 cells is consistent with the predicted expression pattern.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"